focalpair

Merges focal pairs into a single image with partial CTF correction

focalpair <input 1> <input 2> <output> [im1parm=<parm 1>] [im2parm=<parm 2>] [im1=<ctfparm entry>] [im2=<ctfparm entry>] sffile=<SF File> [noampc] [filt=<%>] [noali]

Parameters:


<input 1>First input (MRC only!)
<input 2>Second input (MRC only!)
<output>Combined output
[im1parm=<parm 1>]Parameters of first image (from ctfit)
[im2parm=<parm 2>]Parameters of second image
[im1=<ctfparm entry>]Label from ctfparm.txt file
[im2=<ctfparm entry>]Same for second image
sffile=<SF File>Structure factor file (intensity vs s, 2 column)
[noampc]Do not perform amplitude correction or Wiener filtration
[filt=<%>]High-pass filter the output, 0-100 as a % of Fourier space
[noali]This indicates that the input images have already been aligned to each other

Usage:

fpmerge jj1000.mrc jj1001.mrc jj1000m.mrc sffile=groel.xray

Description

This program impliments focal pair merging for the purpose of combining the low resolution information from a second exposure with the higher resolution information from the first exposure. It performs partial CTF correction. That is, it properly weights the two data sets during combination, and performs phase flipping. However, it does not deconvolute the actual CTF. Instead, it writes an output file containing the necessary information for complete CTF correction at a later point.

The filt option (confusingly) specifies a filter radius as a percentatge. A typical value for the filt option would be ~5, but this may vary for different microscopes/specimens.


EMAN Manual page, generated Wed Feb 18 10:51:32 2009