powdcmp

This will determine a set of orthogonal basis images from a set of projections

powdcmp <images> <basis> [n=<n basis>] [mask=<radius>]

Parameters:


<images>Input file, images to be analyzed
<basis>Output file, set of orthogonal basis images
[n=<n basis>]Number of basis images to output (<= number of input images)
[mask=<radius>]Mask radius in pixels (use the smallest reasonable value)

Usage:

powdcmp proj.hed basis.hed

Description

This program is designed to produce a set of orthogonal basis images to use for noise reduction on raw particles. Typically this program will be used on a set of projections covering the asymmetric triangle, but with no in-plane rotation. Unlike geodcmp, this program will sequence the images in order of dissimilarity. In addition, each projection is aligned to the existing basis set. This alignment should deal with the problem of a single particle orientation having several possible 'best' orientations when using total projected power as an orientation measure.


EMAN Manual page, generated Wed Feb 18 10:51:32 2009