Feature #596
openace2 on FSU Krios
0%
Description
I can't get ace2 to work consistently with images from our Krios. The problem, I believe, is that the edge detection fails on the high mag. images that we are taking. The zeros are relatively close to the origin, and ace2 seems to miss them. When this happens, it misestimates the astig and doesn't elliptically average correctly. I think the best fix for this would be to make an option where you can turn off the edge detection and just circularly average. I don't know who to make a watcher on this, so if I have put the wrong people, please drop yourselves and add the correct ones.
Updated by Neil Voss almost 14 years ago
If edge detection fails it uses circular average. As a temporary workaround, make the edge threshold really high like 0.5 instead of 0.001 and it should work like you want.
Updated by Neil Voss almost 14 years ago
- Target version set to Appion/Leginon Future Version
Updated by Scott Stagg almost 14 years ago
That definitely helped, but it still fails on a large percentage of images. What percentage of images does it succeed on at Scripps?
Updated by Neil Voss almost 14 years ago
It usually works on 100% of any image you would expect it to work, but it was really designed around Tecnai 2. I hope to dive into the code, fix it so it works on more microscopes and then eventually publish it.
Updated by Anchi Cheng almost 14 years ago
Try use Binning option in runAce2.php
I put some behavior description in the pop-up help. In your case, by using a large binning, you zoom into the center of the power spectrum so that ace2 can find the modulation in the range it can handle.
My data are normally at 100kx on Tecnai2 so I have similar problem.