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Feature #708

open

Allow importing old alignment values into SPIDER ref-based alignment

Added by Dmitry Lyumkis almost 14 years ago. Updated almost 14 years ago.

Status:
New
Priority:
Low
Assignee:
-
Category:
-
Start date:
06/24/2010
Due date:
% Done:

0%

Estimated time:

Description

something we've been talking about for a long time:

currently, we are only able to do a single alignment iteration for any stack. One way we can do an iterative alignment is by creating a link button for every aligned stack that would upload it to the database as an original stack. This way, once an aligned stack has been created, the user has the option of uploading it as an "original" stack, then realigning it to get a finer result.

this has some caveats. For example, each program has it's own way of monitoring previous alignment parameters before applying the new ones. SPIDER needs a formatted doc file, IMAGIC stores the information in the headers (which gets lost, by the way, when we reprocess the stack with, for example, EMAN), etc. Probably what we would need to do is create a separate, Appion-specific doc file for each alignment iteration, then translate it to the program-specific parameter file.

further ideas are welcome

Actions #1

Updated by Neil Voss almost 14 years ago

Implementing it is okay, but I would be opposed to using this on my data. It does introduce bias from previous run. My suggestion would be to run an alignment create better templates and then run it again on the original particles with new templates. Most alignments already have iterative alignment built-in, so this would only be useful for alignments between packages or when changing templates.

Actions #2

Updated by Neil Voss almost 14 years ago

Doc files are BAD!!! That is why we have a database!

Actions #3

Updated by Neil Voss almost 14 years ago

  • Subject changed from allow for iterative alignment within appion to Allow importing old alignment values into SPIDER ref-based alignment
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