CTF Estimation » History » Version 4
Anke Mulder, 05/18/2010 07:01 PM
| 1 | 1 | Amber Herold | h1. CTF Estimation |
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| 3 | 4 | Anke Mulder | Regardless of the method eventually utilized for 3D reconstruction, a good starting point for single particle EM investigations is 2D alignment and classification of the dataset. This type of analysis intimately acquaints the scientist with the types of particles, distribution of views, and the relative amount of "junk" contained in the dataset. |
| 4 | 1 | Amber Herold | |
| 5 | 4 | Anke Mulder | h2. Available CTF Procedures: |
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| 7 | 3 | Amber Herold | # [[Ace Estimation]] |
| 8 | # [[Ace 2 Estimation]] |
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| 9 | 1 | Amber Herold | # [[CtfTilt Estimation]] |
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| 12 | 4 | Anke Mulder | h2. Appion SideBar Snapshot: |
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| 14 | !http://emg.nysbc.org/attachments/196/Picture_85.png! |
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| 16 | h2. Appion Composite Output Snapshot: |
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| 18 | !http://emg.nysbc.org/attachments/197/Picture_84.png! |
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| 21 | [[Particle Selection|< Particle Selection]] | [[Stacks|Create Particle Stack >]] |
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| 22 | 1 | Amber Herold | |
| 23 | ______ |