Appion

h1. Install EM Hole Finder

The "EM Hole Finder" package is used for the Auto-masking feature of Appion. It automatically detects carbon edges in micrographs and creates masks for the images to hide the carbon portions. These masks can then be used to exclude particles in those regions from particle stacks.   

h2. Download em_hole_finder package

The package is available at

https://github.com/hbradlow/em_hole_finder

h2. Set up a separate environment to run em_hole_finder

em_hole_finder uses a version of numextension which is not compatible with the rest of Appion. When the em_hole_finder program is called from Appion, it must first run an activation script to set up an appropriate environment for running em_hole_finder.

h2. Set environment variables

To call em_hole_finder from Appion, the installed package directory (em_hole_finder) needs to be in the user's PATH variable.

Also, you need to set an environment variable "HOLE_FIND_ACTIVATE" to the path to the activate script. An example of the path is "/opt/em_hole_finder/env/bin" where a script called activate is located in the bin directory.

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[[Install Protomo|< Install Protomo]] | [[Test Appion|Test Appion >]]

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