Upload Particles¶

Users can upload particle picks from doing a manual picking session outside of appion using EMAN's boxer program.

General Workflow:¶

To launch:

1. User has to make sure that the particle selection file (*.box) has the same name as the micrography the particles are picked from (e.g. if the name of the micrograph is test.mrc, make sure that the particle selection file is called test.box).
2. Alternatively, a text file where particles are list in the style of downloaded particle picks from Appion can be used.
3. Provide the full path to which the selection file(s) is located (Note: wild cards are accepted for *.box).
4. Provide the diameter of the particle
5. If the images from which the particles are picked are binned relative to the images in the database, then provide the scaling value.

Output:

1. The output of the particle selection will be visible under the "Particle Selection" menu

Notes, Comments, and Suggestions:¶

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