Do and Do Not in leginon » History » Version 1
Amber Herold, 04/23/2010 09:07 AM
| 1 | 1 | Amber Herold | h1. Do and Do Not in leginon |
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| 5 | * DO NOT use low dose mode unless "Manual" application is used. |
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| 8 | * DO wait for > 1/2 hr, with scope in high mag HM mode, after LM atlas is |
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| 9 | acquired. |
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| 12 | * DO check and align microscope in both HM and LM mode well. |
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| 15 | * DO wait for targets to finish processing and Do refresh them on the atlas in "Square |
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| 16 | Targeting" Node before selecting and submitting new ones. |
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| 19 | * DO use the "Pause" tool in nodes such as "Square," "Hole," and in Maunal Check window |
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| 20 | of "Z Focus" and "Focus"to pause MSI and adjust microscopy parameters directly. |
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| 23 | * DO NOT move the stage at the microscope while Leginon pauses for user verification of |
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| 24 | "Exposure Targeting" without using Queuing option on the node. If you do so, click the |
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| 25 | "Declare Drift" button " ! " in "Target Adjustment" node. |