Exposure Targeting Set-up for MSI-T for Four holes » History » Revision 8
Revision 7 (Anchi Cheng, 08/16/2016 03:49 PM) → Revision 8/14 (Anchi Cheng, 08/16/2016 03:52 PM)
h1. Exposure Targeting Set-up for MSI-T for Four holes A good way to improve the throughput if there are multiple holes visible in a hl image is to target all four holes. We can set up for 4 holes with this more challenging example: h2. About this image: This image shows the edge of the grid bar. The four holes are reasonably centered. !original.png! !original.png! h2. Leginon/Exposure Targeting/Template> * Cross correlation works better than phase correlation in this case because the hole does not have sharp edge and not perfectly round. * Adjust the value to fill the mean before correlation to minimize the correlation along the edge of grid bar. * Invert template for correlation if needed. !template_corr_settings.png! !template_corr.png! h2. Leginon/Exposure Targeting/Threshold> * Fairly easy to set. !threshold_settings.png! !threshold.png! h2. Leginon/Exposure Targeting/Blobs> * Allow more blobs than the intended 4. !blobs_settings! !blobs.png! h2. Leginon/Exposure Targeting/Lattice> * Similar to that in Hole Targeting/Lattice step. ** Make measurement of the lattice distance ** Estimate the reference intensity for ice thickness filter. !lattice_settings.png! !threshold_with_lattice_measurement.png! !lattice.png! h2. Leginon/Exposure Targeting/Acquisition> * Set up focus template but DO NOT eliminate focus targets using ice thickness criteria. This works because Leginon (3.1 and above) will automatically choose the center most focus target. !acquisition_settings.png! !acquisition_all_focuses.png!