Sub-3Å proteasome stage-shift data collection » History » Revision 5
Revision 4 (Anchi Cheng, 08/16/2016 04:43 PM) → Revision 5/7 (Anchi Cheng, 08/16/2016 05:19 PM)
h1. Sub-3Å proteasome stage-shift data collection h2. Instruments # Scope: FEI Titan-Krios # Camera: Gatan K2. mount directly. 1.21 Å at 29kx scope nominal mag h2. Grid Preset Design * 1.2/1.3 C-flat carbon support; 400 mesh Copper grid. {{include(Krios-K2-TSRI)}} h2. Application: MSI-T2 Advanced (= MSI-T + "Beam Tilt Image" for coma-free alignment + "N2 Filling" for Krios auto nitrogen filler) h2. Presets Manager: No preset cycling (Krios has its own normalization). h2. Queuing usage: Hole Targeting: Queuing, target at the carbon area to cover four holes. Exposure Targeting: Queuing. manual targeting. h2. Move method to reach the targets: Hole: Presets Manager stage position movement Focus: Presets Manager stage position movement Exposure: [[Iterative_Stage_Movement|Navigator Iterative movement]]: Target Tolerance: 1e-7 m; Acceptable Tolerance: 3e-7 m h2. Wait time before final exposure: 40 seconds