Sub-3Å proteasome stage-shift data collection » History » Version 6
Anchi Cheng, 08/16/2016 05:24 PM
| 1 | 1 | Anchi Cheng | h1. Sub-3Å proteasome stage-shift data collection |
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| 3 | h2. Instruments |
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| 5 | # Scope: FEI Titan-Krios |
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| 6 | # Camera: Gatan K2. mount directly. 1.21 Å at 29kx scope nominal mag |
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| 8 | 5 | Anchi Cheng | h2. Grid |
| 9 | 1 | Anchi Cheng | |
| 10 | 5 | Anchi Cheng | * 1.2/1.3 C-flat carbon support; 400 mesh Copper grid. |
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| 12 | 1 | Anchi Cheng | {{include(Krios-K2-TSRI)}} |
| 13 | 5 | Anchi Cheng | |
| 14 | h2. Application: |
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| 15 | |||
| 16 | MSI-T2 Advanced (= MSI-T + "Beam Tilt Image" for coma-free alignment + "N2 Filling" for Krios auto nitrogen filler) |
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| 18 | h2. Presets Manager: |
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| 20 | No preset cycling (Krios has its own normalization). |
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| 22 | h2. Queuing usage: |
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| 24 | Hole Targeting: Queuing, target at the carbon area to cover four holes. |
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| 25 | Exposure Targeting: Queuing. manual targeting. |
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| 27 | h2. Move method to reach the targets: |
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| 29 | 6 | Anchi Cheng | Hole: Presets Manager (modeled) stage position movement |
| 30 | Focus: Presets Manager (modeled) stage position movement |
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| 31 | 5 | Anchi Cheng | Exposure: [[Iterative_Stage_Movement|Navigator Iterative movement]]: Target Tolerance: 1e-7 m; Acceptable Tolerance: 3e-7 m |
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| 33 | h2. Wait time before final exposure: |
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| 35 | 40 seconds |