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Error acquiring image: Error sending request: Request

Added by Caleigh Azumaya over 2 years ago

Hi,

We have been using leginon 3.5 for the last 6 months and things were working until I updated SerialEM on our K3 computer to version 3.8.12 a few days ago. The SEMCCD...dll version was updated and I am having problems with Leginon (though not SerialEM). I downgraded this dll file back down to 2.31-64, but am still getting the same error.

The error is "Error acquiring image: Error sending request: Request" in the GUI log.

It seems like Leginon can "simulate target" to take images fine, but will give the error in all automated nodes like 'Correction' or 'Z-focus' or 'Drift Monitor' and 'Focus'.

The test1.py - test2.py still works ok with the microscope and the client and the camera computer is seemingly registering the correct image sizes when it's throwing the errors.

Does someone know why this would be happening? Thanks for the help.


Replies (7)

RE: Error acquiring image: Error sending request: Request - Added by Anchi Cheng over 2 years ago

Let me ask David Mastronarde what he changed. Can you run a basic pyscope camera test from python command line as described in Gatan_K3_installation_and_setup ? That should have more information on the error. Thanks.

RE: Error acquiring image: Error sending request: Request - Added by Caleigh Azumaya over 2 years ago

Hi Anchi,

Thank you so much. The pyscope test is attached below. Let me know if you need anything else to help diagnose

Caleigh

pyscope_090321[96].png (48.2 KB) pyscope_090321[96].png

RE: Error acquiring image: Error sending request: Request - Added by Anchi Cheng over 2 years ago

The result is normal. However, this is not saving frames. Could you insert the command

k.setSaveRawFrames(True)

before getImage is called ?

RE: Error acquiring image: Error sending request: Request - Added by Anchi Cheng over 2 years ago

I noticed that your original message said that it only happens on nodes such as Correction node. That is particularly odd. Could you give me more details such as settings used and tool used (raw/corrected, channel etc.). A screenshot of the log shown surrounding the error (please expand the window so I can see more), would be helpful, too.

RE: Error acquiring image: Error sending request: Request - Added by Caleigh Azumaya over 2 years ago

Oh yes, specifically, it will take
Raw, Channel 0, Acquire = no error
Dark, Both Channels, Acquire = error
then it will freeze the correction node, but I can move to Hole or Exposure or something and still simulate target to save an image. I will attach a photo in a moment.

RE: Error acquiring image: Error sending request: Request - Added by Caleigh Azumaya over 2 years ago

Well, now there are errors at different places.
Here is a screenshot of the error log and the client log after running
Correction: Raw: Channel 0: Acquire
Correction: Dark: Both Channels: Acquire
Correction: Bright: Both Channels: Acquire
Correction: Corrected: Channel 0: Acquire

There is no error when I do Hole: Simulate Target
But then there are errors (attached) when I try to submit two exposure targets and a focus target (and then submit to run the drift monitor, focus, exposure automatically). Drift monitor gets stuck and will not progress from there.

Download all files
client_log[27].png (118 KB) client_log[27].png client log
leginon_gui_driftmonitor[72].png (705 KB) leginon_gui_driftmonitor[72].png drift monitor error
leginon_gui_log[24].png (404 KB) leginon_gui_log[24].png correction error
leginon_gui_hole_simulatetarget[12].png (591 KB) leginon_gui_hole_simulatetarget[12].png no error
leginon_gui_log2[91].png (542 KB) leginon_gui_log2[91].png correction error 2
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