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Bug #4481

closed

ctffind 3 not working

Added by William Rice over 7 years ago. Updated over 7 years ago.

Status:
Closed
Priority:
Normal
Assignee:
Category:
-
Target version:
Start date:
09/29/2016
Due date:
% Done:

0%

Estimated time:
Affected Version:
Appion/Leginon 3.3
Show in known bugs:
No
Workaround:

Description

ctffind3 seems to be looking for phase shift data.
Session 16sep29f, run ctffindrun9
error:

. def_1=2.3e-06, def_2=2.0e-06, astig_angle=51.9, cross_corr=0.089, 
... reading MRC: 16sep29f_02en-pow.mrc size:(512, 512) dtype:float32 
... writing JPEG: 16sep29f_02en-pow.jpg 
 ==== Committing data to database ====  
... Committing ctf parameters for 16sep29f_02en to database 
!!! WARNING: |def1| > |def2|, flipping defocus axes 
Found 1 ctf values 
0.03619 -- ctffind4run1 
*** 0.036 
Traceback (most recent call last): 
File "/opt/myamisnap/bin/ctfestimate.py", line 465, in  
imgLoop.run() 
File "/opt/myamisnap/lib/appionlib/appionLoop2.py", line 95, in run 
self.loopCommitToDatabase(imgdata) 
File "/opt/myamisnap/lib/appionlib/appionLoop2.py", line 153, in loopCommitToDatabase 
return self.commitToDatabase(imgdata) 
File "/opt/myamisnap/bin/ctfestimate.py", line 367, in commitToDatabase 
ctfinsert.validateAndInsertCTFData(imgdata, self.ctfvalues, self.ctfrun, self.params['rundir']) 
File "/opt/myamisnap/lib/appionlib/apCtf/ctfinsert.py", line 43, in validateAndInsertCTFData 
ctfvalues = runCTFdisplayTools(imgdata, ctfvalues, opimagedir, fftpath, fftfreq) 
File "/opt/myamisnap/lib/appionlib/apCtf/ctfinsert.py", line 83, in runCTFdisplayTools 
ctfdisplaydict = ctfdisplay.makeCtfImages(imgdata, ctfvalues, fftpath, fftfreq) 
File "/opt/myamisnap/lib/appionlib/apCtf/ctfdisplay.py", line 1131, in makeCtfImages 
ctfdisplaydict = a.CTFpowerspec(imgdata, ctfdata, fftpath, fftfreq, twod=twod) 
File "/opt/myamisnap/lib/appionlib/apCtf/ctfdisplay.py", line 969, in CTFpowerspec 
self.extra_phase_shift = to_float(ctfdata['extra_phase_shift']) 
KeyError: 'extra_phase_shift' 


Related issues 1 (0 open1 closed)

Related to Appion - Feature #4489: Add phase plate options to GCTFClosedCarl Negro09/30/2016

Actions
Actions #1

Updated by Anchi Cheng over 7 years ago

  • Project changed from Leginon to Appion
  • Status changed from New to In Test
  • Assignee set to William Rice
  • Target version set to Appion/Leginon 3.3
  • Affected Version changed from Appion/Leginon 3.2 to Appion/Leginon 3.3

Fixed. Please check tomorrow.

Actions #2

Updated by Anchi Cheng over 7 years ago

  • Related to Feature #4489: Add phase plate options to GCTF added
Actions #3

Updated by William Rice over 7 years ago

  • Status changed from In Test to Closed

Working again now. Thanks!

Actions

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