Upload Particles » History » Version 9
Anchi Cheng, 05/29/2013 02:20 PM
1 | 1 | Amber Herold | h1. Upload Particles |
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3 | 2 | Pick-Wei Lau | Users can upload particle picks from doing a manual picking session outside of appion using EMAN's boxer program. |
4 | 7 | Anke Mulder | !http://emg.nysbc.org/attachments/259/Picture_48.png! |
5 | 2 | Pick-Wei Lau | |
6 | 6 | Anke Mulder | h2. General Workflow: |
7 | 1 | Amber Herold | |
8 | 6 | Anke Mulder | *To launch:* |
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10 | 2 | Pick-Wei Lau | # User has to make sure that the particle selection file (*.box) has the same name as the micrography the particles are picked from (e.g. if the name of the micrograph is test.mrc, make sure that the particle selection file is called test.box). |
11 | 9 | Anchi Cheng | # Alternatively, a text file where particles are list in the style of downloaded particle picks from Appion can be used. |
12 | # Provide the full path to which the selection file(s) is located (Note: wild cards are accepted for *.box). |
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13 | 4 | Pick-Wei Lau | # Provide the diameter of the particle |
14 | 1 | Amber Herold | # If the images from which the particles are picked are binned relative to the *images in the database*, then provide the scaling value. |
15 | 2 | Pick-Wei Lau | |
16 | 6 | Anke Mulder | *Output:* |
17 | 2 | Pick-Wei Lau | |
18 | 1 | Amber Herold | # The output of the particle selection will be visible under the "Particle Selection" menu |
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21 | 6 | Anke Mulder | h2. Notes, Comments, and Suggestions: |
22 | 1 | Amber Herold | |
23 | 6 | Anke Mulder | !http://emg.nysbc.org/attachments/277/Picture_81.png! |
24 | 1 | Amber Herold | |
25 | ______ |
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26 | 6 | Anke Mulder | |
27 | 8 | Anke Mulder | [[EMDB to Model|< EMDB to Model]] | [[Upload Template|Upload Template >]] |