How to trouble shoot after the fact when tracking at high tilt went bad while using MSI- Tomography
Added by Anchi Cheng over 13 years ago
Posting for Morgan Beeby,
I ran a Leginon >> tomography session over the weekend, which went very smoothly except for >> one thing: at high tilts on both sides of the tilt series (> ~55 >> degrees), Leginon began to fail to track it's target. Could you point me >> in the right direction to troubleshoot this? In my previous experience, >> it's never done this before.
Replies (1)
RE: How to trouble shoot after the fact when tracking at high tilt went bad while using MSI- Tomography - Added by Anchi Cheng over 13 years ago
The first thing to do is examine the graphs in the Leginon tomography web page where it shows the predictions and actual position during the run. Look for common pattern on the failed runs. There are a number of examples illustrating our common problems under Troubles_with_Tomography. Please post an example of yours here if you need help reading your result.
The next thing to do is to simulate the tilt series run with MSI-SimuTomography application. See Using "MSI-SimuTomography" Application for debugging for instruction. The simulation is helpful when you suspect that Leginon found wrong correlation peaks. If you have trouble understand the printout in the terminal, try capturing that in a file and post it here. I will take a look at it.
If Leginon fails to find the correct correlation peak no matter what parameters you try, you might need to post here the failing pair of images. If it is more than a pair, I will have to write a script for you to do individual testings or we will have to arrange a way for me to download them.
Anchi