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Feature #5182

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Ability to check focus after every 5 or 10 deg tilts in MSI-Tomography and view tilt axis in leginon for MSI-Tomography

Added by Saikat Chowdhury about 7 years ago.

Status:
New
Priority:
High
Assignee:
Category:
Leginon subsystem
Start date:
08/30/2017
Due date:
% Done:

0%

Estimated time:
Deliverable:

Description

Can a feature be added to MSI-Tomography which gives the option for checking focus every 5 or 10 deg tilt increments during tilt series acquisition? According to people collecting tilt series using Serial EM, this feature exists in that program. It will probably require the stage to go back to zero degree and check focus and then go back to its tilted state and continue collecting data for another 5 or 10 deg and repeat checking focus. This is for making sure no dramatic change in the nominal set defocus is taking place during the complete tilt series acquisition.

In addition to this is there a way in Leginon to show the tilt axis. Its not an absolute necessity, but an easy way to place focus targets.

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