Leginon

h1. Acquiring summed frame image thru Leginon

h2. Camera Configuration

Refer to [[DE-12_introduction#Camera_Settings_for_DE-12|introduction]] on the options

# Select DE12 as the camera

# Uncheck "save raw frames"

# Leave "Frames to use" empty to sum up all frames

h2. acquire [[Bright and Dark reference images]]

** Leginon does not research references by frame rate and sensor temperature.  If you change either of them, you will need to acquire new references.

** Use valid exposure time (multiple of frame time length)

** Use long positive readout delay time (>= 2 frame length) to ensure uniform intensity readout on the first recorded frame.

** It does not matter whether you save raw frames or not in this process.  Leginon does not use them for corrections.

# Select DE12 as the camera, configure and uncheck "save raw frames" in the camera configuration of the preset that you want to use