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Adding DE-12 to Leginon » History » Revision 14

Revision 13 (Anchi Cheng, 02/02/2016 02:30 PM) → Revision 14/16 (Anchi Cheng, 09/28/2016 07:32 AM)

h1. Installation Guide for DE-12 or DE-20 

 h2.    DE-12 is on a computer separated from the microscope 

 Please read [[Using_Leginon_on_a_system_where_the_microscope_and_camera_are_controlled_by_different_computers]] first. 

 h2. Extra Package and Installation 

 |_.program/package|_.notes| 
 |DirectElectronAPI.exe|(Should come with the camera)| 
 |"Google protobuf":http://code.google.com/p/protobuf/|| 

 {{include(portobuf installation instruction)}} 



 h2. instruments.cfg 

 {{include(Instrument.cfg for DE-12)}} 
 height and width should be set according to the model. 

 h2. orientation setup 

 Change the orientation in pyscope/de.py for frame orientation definition used in movie frame processing. 

 <pre> 
 # frame flipping configuration. 
 # default here is for DE-12 orientation on FEI F20 
 FRAME_LR_FLIP = True 
 # frame rotate configuration. multiple of 90 degrees if needed 
 # after the flip.    Direction + is x to -y. 
 FRAME_ROTATE = 0 
 </pre< 

 Known values: 
 DE-12 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=0 
 DE-20 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=-1 

 h2. Setup 

 * Create the folder to store the dose fractionation raw frames.    Default location is D:\\frames. 
 * Setup [[DDD_raw_frame_file_transfer|raw frame file transfer]] from a network data server. 

 h2. Testing 

 

 h2. Testing with pyscope 

 {{include(DE-12 Installation Pyscope Test)}} 

 

 h2. Programs to open before Leginon Client: DE Server 

 See how to use DE camera usage in 

 * [[DE-12 introduction]] Please read this!!! 
 * [[Acquiring summed frame image thru Leginon]] 
 * [[DDD raw frame saving|Triggering raw frame saving]] 
 * [[DDD raw frame file transfer|Transferring raw frame images]] off the solid-state drive of DE-12 during data collection to a network drive. 
 * [[Make DE-12 movie stack|Compiling corrected movie stack]] with Appion using bright and dark images acquired in Leginon