Adding DE-12 to Leginon » History » Revision 14
Revision 13 (Anchi Cheng, 02/02/2016 02:30 PM) → Revision 14/16 (Anchi Cheng, 09/28/2016 07:32 AM)
h1. Installation Guide for DE-12 or DE-20 h2. DE-12 is on a computer separated from the microscope Please read [[Using_Leginon_on_a_system_where_the_microscope_and_camera_are_controlled_by_different_computers]] first. h2. Extra Package and Installation |_.program/package|_.notes| |DirectElectronAPI.exe|(Should come with the camera)| |"Google protobuf":http://code.google.com/p/protobuf/|| {{include(portobuf installation instruction)}} h2. instruments.cfg {{include(Instrument.cfg for DE-12)}} height and width should be set according to the model. h2. orientation setup Change the orientation in pyscope/de.py for frame orientation definition used in movie frame processing. <pre> # frame flipping configuration. # default here is for DE-12 orientation on FEI F20 FRAME_LR_FLIP = True # frame rotate configuration. multiple of 90 degrees if needed # after the flip. Direction + is x to -y. FRAME_ROTATE = 0 </pre< Known values: DE-12 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=0 DE-20 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=-1 h2. Setup * Create the folder to store the dose fractionation raw frames. Default location is D:\\frames. * Setup [[DDD_raw_frame_file_transfer|raw frame file transfer]] from a network data server. h2. Testing h2. Testing with pyscope {{include(DE-12 Installation Pyscope Test)}} h2. Programs to open before Leginon Client: DE Server See how to use DE camera usage in * [[DE-12 introduction]] Please read this!!! * [[Acquiring summed frame image thru Leginon]] * [[DDD raw frame saving|Triggering raw frame saving]] * [[DDD raw frame file transfer|Transferring raw frame images]] off the solid-state drive of DE-12 during data collection to a network drive. * [[Make DE-12 movie stack|Compiling corrected movie stack]] with Appion using bright and dark images acquired in Leginon