Adding DE-12 to Leginon » History » Revision 15
Revision 14 (Anchi Cheng, 09/28/2016 07:32 AM) → Revision 15/16 (Anchi Cheng, 09/28/2016 07:33 AM)
h1. Installation Guide for DE-12 or DE-20
h2. DE-12 is on a computer separated from the microscope
Please read [[Using_Leginon_on_a_system_where_the_microscope_and_camera_are_controlled_by_different_computers]] first.
h2. Extra Package and Installation
|_.program/package|_.notes|
|DirectElectronAPI.exe|(Should come with the camera)|
|"Google protobuf":http://code.google.com/p/protobuf/||
{{include(portobuf installation instruction)}}
h2. instruments.cfg
{{include(Instrument.cfg for DE-12)}}
height and width should be set according to the model.
h2. orientation setup
Change the orientation in pyscope/de.py for frame orientation definition used in movie frame processing.
<pre>
# frame flipping configuration.
# default here is for DE-12 orientation on FEI F20
FRAME_LR_FLIP = True
# frame rotate configuration. multiple of 90 degrees if needed
# after the flip. Direction + is x to -y.
FRAME_ROTATE = 0
</pre> </pre<
Known values:
DE-12 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=0
DE-20 on Tecnai F20: FRAME_LR_FLIP = True, FRAME_ROTATE=-1
h2. Setup
* Create the folder to store the dose fractionation raw frames. Default location is D:\\frames.
* Setup [[DDD_raw_frame_file_transfer|raw frame file transfer]] from a network data server.
h2. Testing
h2. Testing with pyscope
{{include(DE-12 Installation Pyscope Test)}}
h2. Programs to open before Leginon Client: DE Server
See how to use DE camera usage in
* [[DE-12 introduction]] Please read this!!!
* [[Acquiring summed frame image thru Leginon]]
* [[DDD raw frame saving|Triggering raw frame saving]]
* [[DDD raw frame file transfer|Transferring raw frame images]] off the solid-state drive of DE-12 during data collection to a network drive.
* [[Make DE-12 movie stack|Compiling corrected movie stack]] with Appion using bright and dark images acquired in Leginon