An Introduction to Leginon » History » Version 1
Amber Herold, 04/13/2010 01:14 PM
1 | 1 | Amber Herold | |
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2 | h1. An introduction to Leginon |
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8 | |||
9 | h2. What is Leginon System? |
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10 | |||
11 | |||
12 | <abstract> |
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13 | |||
14 | Leginon system include the python-side programs that are writen in python and c, the |
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15 | MySQL database and server, and the mainly php-based image and data viewers on a web |
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16 | server. |
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17 | |||
18 | |||
19 | The python-side programs provide a modular framework for building applications for TEM |
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20 | image acquisition and analysis. Nodes can be connected through abstract events in Leginon's |
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21 | modular architecture design. This gives Leginon the flexibility of application customization |
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22 | at multiple levels. Because nodes can be launched from different machines, Leginon's |
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23 | applications can inherently use distributed memory systems. |
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24 | |||
25 | |||
26 | The MySQL-side database and server keep track of all information (metadata) accompanying |
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27 | the acquired images efficiently. |
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28 | |||
29 | |||
30 | The php-side web server and scripts retrieve information from the database and the file |
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31 | storage system to display both raw information and organized reports. |
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32 | |||
33 | |||
34 | NRAMM development includes the python-side and the php-side scripts. MySQL side uses |
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35 | directly the open-source distribution. |
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36 | |||
37 | |||
38 | The website "http://leginon.scripps.edu/":http://leginon.scripps.edu |
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39 | is the central location for leginon information and links. |
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40 | |||
41 | |||
42 | Once registed for leginon download, the users have access to the " bulletin board":http://emg.nysbc.org/bb.viewforum.php?f=2 where users and |
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43 | developers post their questions and answers. |
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44 | |||
45 | |||
46 | Official bug report and feature request should be entered through "bugzilla":http://emg.nysbc.org/bugzilla/enter_bug.cgi?product=Leginon . |
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47 | |||
48 | </abstract> |
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49 | |||
50 | |||
51 | |||
52 | |||
53 | h2. Terminology |
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54 | |||
55 | |||
56 | |||
57 | |||
58 | h2. Application |
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59 | |||
60 | |||
61 | |||
62 | A Leginon application is image acquisition process that is built of several smaller |
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63 | pieces called 'nodes'. An application defines your preferred scheme for how to acquire |
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64 | images. An application definition includes which nodes to use, how they are connected, and |
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65 | where they are running (possibly distributed across several machines). These three concepts |
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66 | of applications (nodes, events, launchers) are described in more detail below. A typical |
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67 | application involves several stages of image acquisition and image processing. Once an |
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68 | application is designed, it can be used repeatedly for different sessions. An application |
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69 | design can be exported for use on other Leginon installations. |
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70 | |||
71 | |||
72 | |||
73 | |||
74 | |||
75 | h2. Session |
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76 | |||
77 | |||
78 | |||
79 | A session is defined as an execution from start to finish of a Leginon application. All |
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80 | data (images, results, etc.) that are created by Leginon is associated with some session. |
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81 | The first thing a user must do when starting Leginon is create a session, or continue an |
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82 | existing session. |
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83 | |||
84 | |||
85 | |||
86 | |||
87 | |||
88 | h2. Instrument |
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89 | |||
90 | |||
91 | |||
92 | An instrument is the microscope/camera system used for acquiring data during a |
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93 | particular session. The facility at which Leginon is installed may have several different |
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94 | microscopes, each with a unique camera setup. Each system is an instance of an |
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95 | Instrument. |
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96 | |||
97 | |||
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99 | |||
100 | |||
101 | h2. Node |
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102 | |||
103 | |||
104 | |||
105 | Nodes are the building blocks of Leginon applications. Nodes are defined for specific |
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106 | tasks. For instance, an "Acquisition" node is designed to acquire images when it receives |
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107 | targets from another node, which is typically some type of 'TargetFinder' node. Nodes can |
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108 | "publish" the data they create. This means they are making their data public for other nodes |
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109 | to use. The other nodes can "research" to find a specific item of data. Nodes may |
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110 | communicate with each other by generating "events". |
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111 | |||
112 | |||
113 | |||
114 | |||
115 | |||
116 | h2. Event |
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117 | |||
118 | |||
119 | |||
120 | An event is a message sent out from a node to notify other nodes that something of |
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121 | interest has happened. A common example is to announce that some data has been published. |
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122 | Another example is to announce that some process has finished. The declaration of which |
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123 | events are routed between which nodes is part of the application design process. |
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124 | |||
125 | |||
126 | |||
127 | |||
128 | |||
129 | h2. Manager |
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130 | |||
131 | |||
132 | |||
133 | Manager is the master of all nodes in an application. Its existence is usually |
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134 | transparent while running a session, but it is responsible for starting up the application |
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135 | with all its nodes and event bindings. It works behind the scenes to ensure that events are |
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136 | properly distributed throughout the system. |
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137 | |||
138 | |||
139 | |||
140 | |||
141 | |||
142 | h2. Launcher |
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143 | |||
144 | |||
145 | |||
146 | A Launcher is the parent process for a set of nodes. There is typically one launcher |
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147 | running on each machine that you intend to have nodes running on. The assignment of which |
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148 | nodes will be started on a particular launcher is defined as part of the application. |
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149 | |||
150 | |||
151 | |||
152 | |||
153 | |||
154 | h2. Preset |
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155 | |||
156 | |||
157 | |||
158 | A preset is a piece of data which encapsulates the state of an instrument. At any time, |
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159 | the current state of an instrument (magnification, image shifts, camera settings, etc) can |
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160 | be recorded for later use. There is a particular class of Node called the PresetsManager |
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161 | which maintains a list of presets for the current Leginon session. These presets are used by |
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162 | other nodes to set the state of the instrument prior to acquiring an image. This allows for |
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163 | a series of images to be acquired at a consistent state, but possibly different targets (see |
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164 | below). This is very similar to the "Low Dose" system on many microscopes, which consists of |
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165 | a few presets like "Search", "Focus", and "Exposure". The Leginon PresetsManager is a more |
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166 | generalized approach which allows for and unlimited number of presets to be used (like |
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167 | several search presets at different magnifications, or multiple exposure presets at |
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168 | different defocus). |
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169 | |||
170 | |||
171 | |||
172 | |||
173 | |||
174 | h2. Target |
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175 | |||
176 | |||
177 | |||
178 | A target is a location where an image will be acquired. Targets are often selected from |
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179 | existing images (using a TargetFinder node). Acquisition nodes are responsible for |
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180 | interpreting Targets and then acquiring images of them. |
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181 | |||
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183 | |||
184 | |||
185 | |||
186 | |||
187 | |||
188 | |||
189 | |||
190 | h2. Graphical User Interface |
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191 | |||
192 | |||
193 | |||
194 | |||
195 | h2. How to use this manual |
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196 | |||
197 | |||
198 | |||
199 | Many places in the manual, a specific section of a window for a node on a |
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200 | launcher/computer/instrument is mentioned. The first time they are mentioned, the full path |
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201 | is shown as |
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202 | |||
203 | |||
204 | computer/program window/node/subsection of the node to open the setting window/setting |
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205 | window/section in the window> |
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206 | |||
207 | |||
208 | It is assumed that the user knows how to follow this path to the specific section of the |
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209 | window by reading the following graphical user interface (GUI) manual and by a little of |
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210 | practice. A abbreviated path rather than the full path will be shown in the latter steps for |
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211 | the same node or application. |
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212 | |||
213 | |||
214 | |||
215 | |||
216 | |||
217 | h2. Leginon Window |
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218 | |||
219 | |||
220 | |||
221 | The window is divided into sections. |
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222 | |||
223 | |||
224 | * Status bar - Leginon status display such as completion of application |
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225 | loading. |
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226 | |||
227 | |||
228 | |||
229 | !http://emg.nysbc.org/software/leginon/images/images/statusbar.png! |
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230 | |||
231 | |||
232 | |||
233 | * Main Menu - Submenus and options derived from Manager and Launcher |
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234 | |||
235 | |||
236 | |||
237 | !http://emg.nysbc.org/software/leginon/images/images/mainmenu.png! |
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238 | |||
239 | |||
240 | |||
241 | * Node Selector - Selection for the node shown in the toolbar, message log, and node |
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242 | panel. It is also used to indicate warning by turning red for broken node. |
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243 | |||
244 | |||
245 | |||
246 | !http://emg.nysbc.org/software/leginon/images/images/nodeselector.png! |
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247 | |||
248 | |||
249 | |||
250 | * Error indicator !http://emg.nysbc.org/software/leginon/images/icons/error.png! - This icon will appear next to a node (in the Node Selector) that has an |
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251 | error. |
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252 | |||
253 | |||
254 | * Message Log Information indicator !http://emg.nysbc.org/software/leginon/images/icons/info.png! |
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255 | - This icon will appear next to a node (in the Node Selector) that has information in |
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256 | its message log. |
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257 | |||
258 | |||
259 | * User Input indicator !http://emg.nysbc.org/software/leginon/images/icons/userinput.png! - This icon will appear next to a node (in the Node Selector) that |
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260 | requires User input in order to continue processing. |
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261 | |||
262 | |||
263 | * Processing indicator !http://emg.nysbc.org/software/leginon/images/icons/processing/green0.png! - This icon will appear next to a node (in the Node Selector) that |
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264 | currently doing processing. |
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265 | |||
266 | |||
267 | |||
268 | |||
269 | |||
270 | |||
271 | |||
272 | h2. Node Panel |
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273 | |||
274 | |||
275 | |||
276 | |||
277 | |||
278 | * Node Panel - The main display/control panel for the node selected by the node |
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279 | selector. |
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280 | |||
281 | |||
282 | |||
283 | !http://emg.nysbc.org/software/leginon/images/images/nodepanel2.png! |
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284 | |||
285 | |||
286 | |||
287 | * Toolbar - An area containing tools for general configuration and execution of the |
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288 | node as well as some tools for convenience specific for individual node. |
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289 | |||
290 | |||
291 | |||
292 | !http://emg.nysbc.org/software/leginon/images/images/toolbar.png! |
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293 | |||
294 | |||
295 | |||
296 | * Message Log - Expandable/scrollable box showing logged information for the |
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297 | node. |
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298 | |||
299 | |||
300 | |||
301 | !http://emg.nysbc.org/software/leginon/images/images/messagelog.png! |
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302 | |||
303 | |||
304 | |||
305 | |||
306 | |||
307 | |||
308 | |||
309 | |||
310 | h2. Parameter Entry |
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311 | |||
312 | |||
313 | |||
314 | |||
315 | |||
316 | * Free entry box- For typing in either text or numbers. If in a setting window, the |
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317 | altered entry is saved to the database when it is exited with "OK" or after |
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318 | "Apply"/"Test". Most numerical entry accepts floating point number in any python format. |
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319 | For example, 10.2 can also be entered as 1.02e+2. |
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320 | |||
321 | |||
322 | |||
323 | !http://emg.nysbc.org/software/leginon/images/images/Entry_box.png! |
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324 | |||
325 | |||
326 | |||
327 | * Restricted-entry: The user can only select a valid entry from a list. |
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328 | |||
329 | |||
330 | |||
331 | !http://emg.nysbc.org/software/leginon/images/images/Entry_box_restricted.png! |
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332 | |||
333 | |||
334 | |||
335 | * List box- This type of selection box select either an ordered sequence or a simple |
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336 | unordered list. If ordered, the items in the sequence can order with "up" and "down" |
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337 | tools. |
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338 | |||
339 | |||
340 | |||
341 | !http://emg.nysbc.org/software/leginon/images/images/List_box.png! |
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342 | |||
343 | |||
344 | |||
345 | * If ordered, the items in the sequence can be shifted around with "up" and "down" |
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346 | tools. |
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347 | |||
348 | |||
349 | * Either free or restricted entry box is used for entering a new item to the list. |
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350 | "+" tool is used to add the entry to the list box. selecting an existing item and |
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351 | press "-" tool removes the item from the list. |
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352 | |||
353 | |||
354 | * Multiple items can be selected by holding down the "ctrl" or "shift" key. The |
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355 | latter selects a range between the first and the new items. |
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356 | |||
357 | |||
358 | |||
359 | |||
360 | |||
361 | |||
362 | |||
363 | |||
364 | |||
365 | h2. Image Viewing |
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366 | |||
367 | |||
368 | |||
369 | |||
370 | |||
371 | * Display Panel - where the image is shown. |
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372 | |||
373 | |||
374 | * (pixel value button) !http://emg.nysbc.org/software/leginon/images/icons/value.png! - Pixel value display tool that shows the (x,y) coordinate the intensity value at the |
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375 | cursor. The coordinate origin is set at top-left corner. |
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376 | |||
377 | |||
378 | * (ruler button) !http://emg.nysbc.org/software/leginon/images/icons/ruler.png! - Ruler tool that displays the distance between a reference point and the current |
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379 | cursor position. Left click at any position inside the display panel defines the |
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380 | reference point. |
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381 | |||
382 | |||
383 | * (zoom button) !http://emg.nysbc.org/software/leginon/images/icons/zoom.png! |
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384 | - Magnifying glass tool that zoom in (left click) or out (right click) at the location |
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385 | of the cursor. |
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386 | |||
387 | |||
388 | * (zoom selector list) !http://emg.nysbc.org/software/leginon/images/images/zoomselector.png! - Direct zoom factor selector. |
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389 | |||
390 | |||
391 | * (cross hair button) !http://emg.nysbc.org/software/leginon/images/images/bluech.png! - Cross hair reference tool displaying a blue cross hair centered at |
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392 | the center of the image for reference. |
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393 | |||
394 | |||
395 | * (display range selection/input) - Contrast and brightness adjustment of the image |
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396 | display. The default is mean +/- 5*standard deviation of image intensity. This can be |
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397 | modified by setting the minimum (top scroll bar or entry box) and the maximaum (bottom |
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398 | scroll bar or entry box) of the display range. |
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399 | |||
400 | |||
401 | |||
402 | !http://emg.nysbc.org/software/leginon/images/images/contrbright.png! |
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403 | |||
404 | |||
405 | |||
406 | * Image Stats Panel |
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407 | |||
408 | |||
409 | |||
410 | !http://emg.nysbc.org/software/leginon/images/images/imstatpanel.png! |
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411 | |||
412 | |||
413 | |||
414 | * Image/Target/Marker Control Panel - where the image and targets to be displayed in |
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415 | the display panel are determined. Targets are indicated by short cross symbols or other |
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416 | shapes of different colors. The items shown with a screen icon are of image type. Items |
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417 | of image type can be displayed alone while markers will show only when one of the items |
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418 | of image type is also displayed. |
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419 | |||
420 | |||
421 | |||
422 | !http://emg.nysbc.org/software/leginon/images/images/imcontrolpanel.png! |
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423 | |||
424 | |||
425 | |||
426 | * (status indicator) |
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427 | |||
428 | |||
429 | |||
430 | <inlinegraphic |
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431 | fileref="http://emg.nysbc.org/software/leginon/images/icons/green.png" |
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432 | format="PNG"/> |
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433 | <inlinegraphic |
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434 | fileref="http://emg.nysbc.org/software/leginon/images/icons/red.png" |
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435 | format="PNG"/> |
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436 | |||
437 | |||
438 | - This can be either green or red. During a step-by-step testing of parameters, |
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439 | green indicates that the step has been tested and you can proceed to the next |
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440 | testing step. Skiping to a step below a red-indicated step will cause an |
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441 | error. |
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442 | |||
443 | |||
444 | * (image display tool) !http://emg.nysbc.org/software/leginon/images/icons/display.png! - Display selection. Only one of the image type can be displayed at |
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445 | one time. |
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446 | |||
447 | |||
448 | * (target/marker display tool) !http://emg.nysbc.org/software/leginon/images/images/target_display.png! - Display target or marker selection. Pressed down (as shown for |
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449 | the green target) is active. More than one type of markers can be displayed on |
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450 | layers above the image. If the markers are overlapped, the marker last to be |
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451 | activated for display is at the top. |
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452 | |||
453 | |||
454 | * (marker tool) !http://emg.nysbc.org/software/leginon/images/icons/arrow.png! - Marker input selection. Certain markers can be manually edited. |
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455 | In general, these are targets for further acquisition. In such case, the marker |
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456 | selection tool can be activated by left clicking the icon. Once activated, left |
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457 | click in the display panel adds the marker to the image and right click removes the |
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458 | marker pointed to. |
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459 | |||
460 | |||
461 | * (settings) !http://emg.nysbc.org/software/leginon/images/icons/settings.png! - Settings are available for certain items in the image control |
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462 | panel, mainly steps in automatic hole finding. Left click opens the setting window |
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463 | and the behavior of the setting window is similar to others of the same type. |
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464 | |||
465 | |||
466 | |||
467 | |||
468 | |||
469 | |||
470 | |||
471 | |||
472 | |||
473 | h2. Tool Bar |
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474 | |||
475 | |||
476 | |||
477 | Tool bar of the node contains tools for setting and execution of the node functions. In |
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478 | addition, some tools are available as pop-up for convenience. The general rule for the flow |
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479 | of setting up and execution is to go from left to right up to the execution tool (execution |
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480 | icon). |
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481 | |||
482 | |||
483 | |||
484 | !http://emg.nysbc.org/software/leginon/images/images/toolbar.png! |
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485 | |||
486 | |||
487 | |||
488 | |||
489 | |||
490 | |||
491 | h2. Settings Window |
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492 | |||
493 | |||
494 | |||
495 | Settings windows are opened by left clicking its button !http://emg.nysbc.org/software/leginon/images/icons/settings.png! |
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496 | in the toolbar. Note that only one setting swindow can be opened at a time and that all |
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497 | functions in the main leginon window is inactivated when a settings window is open even if |
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498 | the leginon window is the focus. The content of the settings window depends on the purpose |
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499 | of the settings. The general controls are described here: |
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500 | |||
501 | |||
502 | * Test: found in settings of image processing steps. When clicked, the current setting |
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503 | in the window is saved to database and applied to the current input and the results |
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504 | saved as its output. If the item output is displayed in the node display panel, the |
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505 | content will be changed accordingly. |
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506 | |||
507 | |||
508 | |||
509 | !http://emg.nysbc.org/software/leginon/images/images/test.png! |
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510 | |||
511 | |||
512 | |||
513 | * Apply: save the modified setting to database for future execution but not the |
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514 | current test input. It is activated only if the modification of the input is completed, |
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515 | normally by pressing the Enter key on the keyboard. Since current GUI does not allow |
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516 | changes to the main window to be made while setting window is on, Using button is not |
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517 | necessary since "OK" button will save the settings anyway. |
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518 | |||
519 | |||
520 | |||
521 | !http://emg.nysbc.org/software/leginon/images/images/apply.png! |
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522 | |||
523 | |||
524 | |||
525 | * Cancel: close the setting window and revert the setting to the last saved values. |
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526 | This means you can not cancel setting modification if you have "test"ed or "apply"ed the |
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527 | settings. |
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528 | |||
529 | |||
530 | |||
531 | !http://emg.nysbc.org/software/leginon/images/images/cancel.png! |
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532 | |||
533 | |||
534 | |||
535 | * OK or Done: close the setting window and save all modified settings to the |
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536 | database. |
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537 | |||
538 | |||
539 | |||
540 | !http://emg.nysbc.org/software/leginon/images/images/ok.png! |
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541 | |||
542 | |||
543 | |||
544 | |||
545 | |||
546 | |||
547 | |||
548 | |||
549 | h2. Execution tools |
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550 | |||
551 | |||
552 | |||
553 | Found in the node toolbar. They may take on different meanings for different node |
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554 | classes. The general ones are described here: |
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555 | |||
556 | |||
557 | * (Execute/Submit button) !http://emg.nysbc.org/software/leginon/images/icons/play.png! |
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558 | : Perform one of the four possible functions if available and active for a node: |
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559 | |||
560 | |||
561 | * When the application is in standby, it executes, sometimes with setting up the |
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562 | main function of the node. |
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563 | |||
564 | |||
565 | * When the application is running automatically, it indicates that the node is in |
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566 | use. |
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567 | |||
568 | |||
569 | * When the application is paused for user input, it submit the user approved |
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570 | inputs and continue on the flow. |
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571 | |||
572 | |||
573 | * When the application is paused by the "pause" tool in the same node, it removes |
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574 | the pause flag and continue on the application flow. |
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575 | |||
576 | |||
577 | |||
578 | |||
579 | * (Pause button) !http://emg.nysbc.org/software/leginon/images/icons/pause.png! : Pause data processing flow when the current input in a queue list is done. |
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580 | |||
581 | |||
582 | * (Abort button) !http://emg.nysbc.org/software/leginon/images/icons/stop.png! |
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583 | : Abort all data processing of the whole list in the queue. |
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584 | |||
585 | |||
586 | |||
587 | |||
588 | |||
589 | |||
590 | |||
591 | |||
592 | |||
593 | h2. Minimum Requirement and current NRAMM setup |
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594 | |||
595 | |||
596 | |||
597 | *Recommendation is based on the current NRAMM setup |
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598 | |||
599 | |||
600 | |||
601 | h2. Electron Microscope with its Controling Computer |
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602 | |||
603 | |||
604 | |||
605 | FEI Tecnai 12 with Scripting SW, network connected, and the ability to install software |
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606 | on it |
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607 | |||
608 | |||
609 | |||
610 | |||
611 | |||
612 | h2. CCD |
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613 | |||
614 | |||
615 | |||
616 | Gatan, or Tietz-minimal 1kx1k; NRAMM 4kx4k |
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617 | |||
618 | |||
619 | |||
620 | |||
621 | |||
622 | h2. A Second Computer |
||
623 | |||
624 | |||
625 | |||
626 | |||
627 | h2. CPU |
||
628 | |||
629 | |||
630 | |||
631 | Minimal 1 GHz; NRAMM AMD Athlon 64 FX-60 Dual Core 2.6 GHz |
||
632 | |||
633 | |||
634 | |||
635 | |||
636 | |||
637 | h2. RAM |
||
638 | |||
639 | |||
640 | |||
641 | Minimal 1 GB physical memory and 1GB swap; NRAMM 4GB physical memory and 2GB |
||
642 | swap |
||
643 | |||
644 | |||
645 | |||
646 | |||
647 | |||
648 | h2. Hard drive |
||
649 | |||
650 | |||
651 | |||
652 | 10 GB for the softwares and maybe a few hours worth of data collection. Much larger |
||
653 | for routine use. NRAMM 15Tb and growing |
||
654 | |||
655 | |||
656 | |||
657 | |||
658 | |||
659 | h2. Network connection |
||
660 | |||
661 | |||
662 | |||
663 | 100 Mbps; NRAMM 1 Gbps |
||
664 | |||
665 | |||
666 | |||
667 | |||
668 | |||
669 | |||
670 | |||
671 | h2. Leginon system components developed at Leginon home |
||
672 | |||
673 | |||
674 | |||
675 | Leginon Home: "http://www.leginon.org/":http://www.leginon.org/ |
||
676 | |||
677 | |||
678 | |||
679 | |||
680 | |||
681 | h2. Supporting packages and programs available through internet or you Linux |
||
682 | distribution |
||
683 | |||
684 | |||
685 | |||
686 | There are minimum of ten packages or single programs, some of them are included in your |
||
687 | Linux distribution. |
||
688 | |||
689 | |||
690 | |||
691 | |||
692 | |||
693 | |||
694 | |||
695 | h2. Leginon supporting programs available upon request |
||
696 | |||
697 | |||
698 | |||
699 | adaexp.exe that is required if film exposure is to be made through Leginon on FEI Tecnai |
||
700 | machines is available by request. Please contact Max Otten: <email>mto@nl.feico.com</email> |
||
701 | and let him know what version of the Tecnai user interface you are using. |
||
702 | |||
703 | |||
704 | |||
705 | |||
706 | |||
707 | |||
708 | |||
709 | |||
710 | |||
711 | h2. Getting Started |
||
712 | |||
713 | |||
714 | |||
715 | |||
716 | h2. Full (Super User) Manual - Getting Started |
||
717 | |||
718 | |||
719 | |||
720 | These steps are a general overview of walking through the entire procedure involving |
||
721 | installation, microscope alignment, calibrations, and using Leginon applications (e.g. |
||
722 | Manual, Calibrations, MSI). |
||
723 | |||
724 | |||
725 | |||
726 | h2. Download Leginon System |
||
727 | |||
728 | |||
729 | "http://www.leginon.org/":http://www.leginon.org/ |
||
730 | |||
731 | |||
732 | |||
733 | |||
734 | |||
735 | h2. Install Leginon System |
||
736 | |||
737 | |||
738 | |||
739 | Refer to the <link linkend="completeinstall">Complete Installation</link> |
||
740 | |||
741 | |||
742 | |||
743 | |||
744 | |||
745 | h2. Add Users, Groups, Instruments, Calibrations, and Applications |
||
746 | |||
747 | |||
748 | |||
749 | Refer to <link linkend="addusers">Administration</link> |
||
750 | |||
751 | |||
752 | For advanced Leginon users who would like to create an application, see the <link |
||
753 | linkend="createapp">application creation chapter</link>. |
||
754 | |||
755 | |||
756 | |||
757 | |||
758 | |||
759 | h2. Test run |
||
760 | |||
761 | |||
762 | |||
763 | Refer to <link linkend="runleg_chapter">Start Leginon</link> chapter for test run on |
||
764 | the microscope and on a remote computer. |
||
765 | |||
766 | |||
767 | |||
768 | |||
769 | |||
770 | h2. Align the microscope |
||
771 | |||
772 | |||
773 | |||
774 | Refer to <link linkend="microscope_setup">Microscope Set-up</link> |
||
775 | |||
776 | |||
777 | |||
778 | |||
779 | |||
780 | h2. Calibrate Leginon for the microscope |
||
781 | |||
782 | |||
783 | |||
784 | Refer to the <link linkend="calapp_chapter">Calibrations chapter</link> |
||
785 | |||
786 | |||
787 | |||
788 | |||
789 | |||
790 | h2. Set-up and Run MSI |
||
791 | |||
792 | |||
793 | |||
794 | Refer to <link linkend="MISQuickStart">MSI quick set-up checklist</link> |
||
795 | |||
796 | |||
797 | AND |
||
798 | |||
799 | |||
800 | Refer to <link linkend="leg1operation">MSI Operation</link> |
||
801 | |||
802 | |||
803 | |||
804 | |||
805 | |||
806 | h2. Help yourself and others |
||
807 | |||
808 | |||
809 | |||
810 | Refer to <link linkend="Inst_troubleshooting">Installation Trouble Shooting</link>, |
||
811 | <link linkend="MSI_troubleshooting">User Trouble Shooting</link> and Leginon "Bulletin Board":http://emg.nysbc.org/bb/viewforum.php?f=2 . |
||
812 | |||
813 | |||
814 | |||
815 | |||
816 | |||
817 | |||
818 | |||
819 | h2. Know the details |
||
820 | |||
821 | |||
822 | |||
823 | For advanced Leginon users who would like to create an application, see the <link |
||
824 | linkend="createapp">application creation chapter</link>. |
||
825 | |||
826 | |||
827 | Refer to <link linkend="Nodes">Node Description</link> |
||
828 | |||
829 | |||
830 | |||
831 | |||
832 | |||
833 | |||
834 | |||
835 | h2. Installation (System Administrator) - Getting Started |
||
836 | |||
837 | |||
838 | |||
839 | These steps are geared soley towards the system administator(s) that will installing |
||
840 | Leginon and setting it up initially for the rest of the Leginon users that need not be |
||
841 | concerned with these details. |
||
842 | |||
843 | |||
844 | Refer to <link linkend="Inst_troubleshooting">Installation Trouble Shooting</link>, and |
||
845 | Leginon "Bulletin |
||
846 | Board":http://emg.nysbc.org/bb/viewforum.php?f=2 . |
||
847 | |||
848 | |||
849 | |||
850 | h2. Download Leginon System |
||
851 | |||
852 | |||
853 | |||
854 | Leginon Home: "http://www.leginon.org/":http://www.leginon.org/ |
||
855 | |||
856 | |||
857 | Direct links to downloads: |
||
858 | |||
859 | |||
860 | * "http://emg.nysbc.org/software/leginon/":http://emg.nysbc.org/software/leginon/ |
||
861 | |||
862 | |||
863 | * "http://emg.nysbc.org/software/dbemtools/":http://emg.nysbc.org/software/dbemtools/ |
||
864 | |||
865 | |||
866 | * "http://emg.nysbc.org/software/mrctools":http://emg.nysbc.org/software/mrctools . |
||
867 | |||
868 | |||
869 | |||
870 | |||
871 | |||
872 | |||
873 | |||
874 | h2. Install Leginon System |
||
875 | |||
876 | |||
877 | |||
878 | Refer to the <link linkend="completeinstall">Complete Installation</link> |
||
879 | |||
880 | |||
881 | |||
882 | |||
883 | |||
884 | h2. Add Users, Groups, Instruments, Calibrations, and Applications |
||
885 | |||
886 | |||
887 | |||
888 | Refer to <link linkend="addusers">Administration</link> |
||
889 | |||
890 | |||
891 | |||
892 | |||
893 | |||
894 | h2. Test run |
||
895 | |||
896 | |||
897 | |||
898 | Refer to <link linkend="runleg_chapter">Start Leginon</link> chapter for test run on |
||
899 | the microscope and on a remote computer. |
||
900 | |||
901 | |||
902 | |||
903 | |||
904 | |||
905 | |||
906 | |||
907 | h2. Short Manual (Basic Leginon User) - Getting Started |
||
908 | |||
909 | |||
910 | |||
911 | These steps are intended to help the basic Leginon user who will operate Leginon (and |
||
912 | not care about installation or the first-time calibrations). |
||
913 | |||
914 | |||
915 | |||
916 | h2. Align the microscope |
||
917 | |||
918 | |||
919 | |||
920 | Refer to <link linkend="microscope_setup">Microscope Set-up</link> |
||
921 | |||
922 | |||
923 | |||
924 | |||
925 | |||
926 | h2. Recording images with Low Dose Kit on the microscope |
||
927 | |||
928 | |||
929 | |||
930 | Refer to using the <link linkend="manual_app">Manual</link> application |
||
931 | |||
932 | |||
933 | |||
934 | |||
935 | |||
936 | h2. Set-up and run MSI |
||
937 | |||
938 | |||
939 | |||
940 | Refer to <link linkend="MISQuickStart">MSI quick set-up checklist</link> |
||
941 | |||
942 | |||
943 | AND |
||
944 | |||
945 | |||
946 | Refer to <link linkend="leg1operation">MSI Operation</link> |
||
947 | |||
948 | |||
949 | |||
950 | |||
951 | |||
952 | h2. Help yourself and others |
||
953 | |||
954 | |||
955 | |||
956 | Refer to <link linkend="MSI_troubleshooting">User Trouble Shooting</link> |