Aperture selection through tem controller » History » Revision 4
Revision 3 (Anchi Cheng, 04/24/2020 03:42 PM) → Revision 4/5 (Anchi Cheng, 04/24/2020 04:19 PM)
h1. Aperture selection through tem controller
This feature allows selection of aperture of TFS microscopes with automated apertures. The current implementation uses AutoIt Scripting and reads the configuration from c:\Program Files (x86)\myami\fei.cfg
h2. Setup
h3. compile autoit script
The script is pyscope/autoit/ApertureSelection.au3
Use AutoIt suite to compile it. Either 32 or 64-bit version works as far as I know. Save the executable at the place you plan to specify in fei.cfg
h3. Setup pyscope fei.cfg
Copy from pyscope/fei.cfg.template these lines to fei.cfg used on your scope. In standard installation, this should be "c:\Program Files (x86)\myami\fei.cfg"
Modify these settings according to your scope.
<pre>
[aperture]
# Disable control of auto apertures if not working or not available
# not all versions of microscope software allows auto aperture control
# from scripting level.
USE_AUTO_APERTURE = False
# AutoIt Automation
AUTOIT_APERTURE_SELECTION_EXE_PATH = c:\Users\supervisor\Desktop\Anchi\AutoItScripts\ApertureSelection.exe
# Does the Titan column has automated c3 aperture ? This affects the id of the
# gui button for AutoIt script.
HAS_AUTO_C3 = True
# Individual aperture selections. If retractable, add open at the end of the list
# List these in the order of what appears in the TUI
# Note: can not handle multiple apertures of the same name.
CONDENSER_2 = 150, 100, 50, 20
OBJECTIVE = 100, 70, 50, open
# Optional selected area aperture control. comment or remove the line to deactivate it.
SELECTED_AREA = 200,100, 40, 10, open
</pre>
h2. Testing
h3. Testing the AutoIt executable
# Open TEM user interface.
# Retract the objective aperture.
# Double click Autoit executable to run.
If this works right, it should insert the 100 um aperture.
h3. Testing in with python command from pyscope
<pre>
from pyscope import fei
f=fei.Krios() # Change this to match your instrument class
f.getApertureSelections('objective') # This should give you the list you specified in fei.cfg
'100', '70', '50' , 'open'
f.getApertureSelection('objective') # The returned value should be the current aperture position as a string
'100'
f.setApertureSelection('objective', '50') # This should insert the 50 um aperture
f.retractApertureMechanism('objective') # This should retract the aperture as if you click on the Activate/Deactivate aperture button to disable it.
</pre>
h2. Use it in Leginon
The most likely usage of this is in [[TEM_Controller|TEM controller]] TEM controller (aliased as TEM_Remote in MSI application).