Continuous tilt electron diffraction recording¶

Micro-ED involves recording 3D crystal electron diffraction pattern during a continuous stage tilt. Leginon implementation of this application includes the listed components below and is available by using myami-ed branch until it is officially added to future release. As is, it is only fully implemented for TFS Ceta or Ceta-D camera.

Since many of the feature required here are not available through Standard or Advanced TEM Scripting from TFS, AutoIt scripts are used in several places.

Components of the feature¶

1. Diffraction mode TEM instrument
2. Beamstop control
3. Rolling-shutter movie acquisition using TIA interface
4. Data conversion and upload of the movies into Leginon database and crystallography format
5. MSI-diffraction application

Installation (assuming that you've already have Leginon installation).¶

1. Check that you have access to Advanced TEM Scripting (frame-saving upgrade not needed).
2. Install or set environment to use git branch myami-ed on TFS microscope with Ceta camera as well as your leginon linux box.
3. AutoIt program and script compilation
4. import MSI-diffraction application and settings from your_myami/leginon/applications