Continuous tilt electron diffraction recording » History » Revision 11
Revision 10 (Anchi Cheng, 02/08/2020 10:57 PM) → Revision 11/37 (Anchi Cheng, 02/08/2020 11:00 PM)
h1. Continuous tilt electron diffraction recording use Ceta or Ceta-D camera and TFS scopes
Micro-ED involves recording 3D crystal electron diffraction pattern during a continuous stage tilt. Leginon implementation of this application includes the listed components below and is available by using myami-ed branch until it is officially added to future release. As is, it is only fully implemented for TFS Ceta or Ceta-D camera.
Since many of the feature required here are not available through Standard or Advanced TEM Scripting from TFS, AutoIt scripts are used in several places.
h2. Components of the feature
# Diffraction mode TEM instrument
# Beamstop control: achieved by AutoIt Scripts BeamstopIn and BeamstopOut
# Rolling-shutter movie acquisition using TIA interface
# Data conversion and upload of the movies into Leginon database and crystallography format
# [[ MSI-Diffr application ]]
# [[Camera length calibration]]
h2. Installation (assuming that you've already have Leginon installation).
# Check that you have access to Advanced TEM Scripting (frame-saving upgrade not needed).
# Install or set environment to use git branch myami-ed on TFS microscope with Ceta camera as well as your leginon linux box.
# import updated Calibrations application (see [[Steps_involved_in_the_installation]])
# import MSI-diffraction and settings from your_myami/leginon/applications (see general description of [[Steps_involved_in_the_installation]] regarding importing application and additional settings for the application)
# [[Setup Diffraction mode TEM instrument]]
# [[Setup Ceta to use Advanced TEM Scripting]]
# [[AutoIt program and script compilation]]
h2. Usage
(To be written)