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Continuous tilt electron diffraction recording » History » Revision 11

Revision 10 (Anchi Cheng, 02/08/2020 10:57 PM) → Revision 11/37 (Anchi Cheng, 02/08/2020 11:00 PM)

h1. Continuous tilt electron diffraction recording use Ceta or Ceta-D camera and TFS scopes 

 Micro-ED involves recording 3D crystal electron diffraction pattern during a continuous stage tilt.    Leginon implementation of this application includes the listed components below and is available by using myami-ed branch until it is officially added to future release.    As is, it is only fully implemented for TFS Ceta or Ceta-D camera. 

 Since many of the feature required here are not available through Standard or Advanced TEM Scripting from TFS, AutoIt scripts are used in several places. 

 h2. Components of the feature 

 # Diffraction mode TEM instrument 
 # Beamstop control: achieved by AutoIt Scripts BeamstopIn and BeamstopOut 
 # Rolling-shutter movie acquisition using TIA interface 
 # Data conversion and upload of the movies into Leginon database and crystallography format 
 # [[ MSI-Diffr application ]] 
 # [[Camera length calibration]] 

 h2. Installation (assuming that you've already have Leginon installation). 

 # Check that you have access to Advanced TEM Scripting (frame-saving upgrade not needed). 
 # Install or set environment to use git branch myami-ed on TFS microscope with Ceta camera as well as your leginon linux box. 
 # import updated Calibrations application (see [[Steps_involved_in_the_installation]]) 
 # import MSI-diffraction    and settings from your_myami/leginon/applications (see general description of [[Steps_involved_in_the_installation]] regarding importing application and additional settings for the application) 
 # [[Setup Diffraction mode TEM instrument]] 
 # [[Setup Ceta to use Advanced TEM Scripting]] 
 # [[AutoIt program and script compilation]] 

 h2. Usage 

 (To be written)