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Anchi Cheng, 04/29/2020 05:14 PM
Continuous tilt electron diffraction recording use Ceta or Ceta-D camera and TFS scopes¶
Micro-ED involves recording 3D crystal electron diffraction pattern during a continuous stage tilt. Leginon implementation of this application includes the listed components below and is available by using myami-ed branch until it is officially added to future release. As is, it is only fully implemented for TFS Ceta or Ceta-D camera.
Since many of the feature required here are not available through Standard or Advanced TEM Scripting from TFS, AutoIt scripts are used in several places.
Components of the feature¶
- Diffraction mode TEM instrument
- Beamstop control: achieved by AutoIt Scripts BeamstopIn and BeamstopOut
- Rolling-shutter movie acquisition using TIA interface
- Data conversion and upload of the movies into Leginon database and crystallography format
- MSI-Diffr application
- Camera length calibration
Installation (assuming that you've already have Leginon installation).¶
- Check that you have access to Advanced TEM Scripting (frame-saving upgrade not needed).
- Install or set environment to use git branch myami-ed or current myami-beta on TFS microscope with Ceta camera as well as your leginon linux box.
- import updated Calibrations application (see Steps_involved_in_the_installation)
- import MSI-diffraction and settings from your_myami/leginon/applications (see general description of Steps_involved_in_the_installation regarding importing application and additional settings for the application)
- Setup Diffraction mode TEM instrument
- Setup Ceta to use Advanced TEM Scripting
- AutoIt program and script compilation
Usage¶
(To be written)
Updated by Anchi Cheng over 4 years ago · 12 revisions