Continuous tilt electron diffraction recording » History » Revision 5
Revision 4 (Anchi Cheng, 12/05/2019 07:45 PM) → Revision 5/37 (Anchi Cheng, 12/06/2019 02:24 PM)
h1. Continuous tilt electron diffraction recording Micro-ED involves recording 3D crystal electron diffraction pattern during a continuous stage tilt. Leginon implementation of this application includes the listed components below and is available by using myami-ed branch until it is officially added to future release. As is, it is only fully implemented for TFS Ceta or Ceta-D camera. Since many of the feature required here are not available through Standard or Advanced TEM Scripting from TFS, AutoIt scripts are used in several places. h2. Components of the feature # [[ Diffraction mode TEM instrument ]] # [[ Beamstop control: achieved by AutoIt Scripts BeamstopIn and BeamstopOut control ]] # [[ Rolling-shutter movie acquisition using TIA interface ]] # [[ Data conversion and upload of the movies into Leginon database and crystallography format ]] # [[ MSI-Diffr application ]] h2. Installation (assuming that you've already have Leginon installation). # Check that you have access to Advanced TEM Scripting (frame-saving upgrade not needed). # Install or set environment to use git branch myami-ed on TFS microscope with Ceta camera as well as your leginon linux box. # [[AutoIt program and script compilation]] # import MSI-diffraction application and settings from your_myami/leginon/applications (see general description of [[Steps_involved_in_the_installation]] regarding importing application and additional settings for the application) # [[Setup Diffraction mode TEM instrument]] # [[AutoIt program and script compilation]] h2. Usage (To be written)