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Current NRAMM setup » History » Version 2

Anchi Cheng, 09/14/2017 07:56 PM

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h1. Current NRAMM setup (9/14/17)
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NRAMM is located at Simons Electron Microscopy Center (SEMC) at New York Structural Biology Center.
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We currently have 6 TEM.
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1 x JEOL 1230 with Gatan UltraScan 4000
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1 x FEI T-12 with TVIPS F416
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1 x FEI F20 with DE-20 camera and TVIPS F416
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3 x FEI Krios with Falcon |||, Ceta, and Gatan K2 Summit
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All 6 microscopes use Leginon for data collection and Appion for preprocessing (defined as frame alignment/ctf estimation/particle picking) and on the same database server and together has 3 web servers to serve the images for users to view and/or as Appion gui to construct the preprocessing commands.  What is listed does not include infrastructure for 2D/3D classification and other post-processing.
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h2.  Network
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All hardware mentioned below as well as the instruments are on a local 10 GB copper network.
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h2. Leginon control or processing work station
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One station per scope. For example,
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CPU 8 x Intel i7-6700 @ 3.40GHz
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MemoryL 16 GB
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Storage: 50G HDD
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h2. Database server
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Dell R730 with (2) Intel E5-2689 3.1GHz, lOCore CPUs,
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Memory: 128GB of RAM,
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Storage: (2) 120GB HDD, (1) 800GB SSD
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h2. Web server
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1. For local network only
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* CPU 4 x Intel Xeon @ 3.00GHz
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* Memory: 4 GB
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* Storage 700GB HDD
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2. For local network only (This is one of the node on our processing cluster.  Rather under used this way)
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* CPU 24 Intel Xeon E5-2670 v3 @2.30GHz
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* Memory: 265 MB
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3. Internet accessible: for guests and member institution to use from outside the building
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* CPU: 8 x Intel Xeon L5410 @ 2.33GHz
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* Memory: 8 GB + 8 GB swap
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h2. Frame processing server (One per microscope)
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h3. K2/Falcon |||
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An injection or buffer server to process frames before it goes to gpfs primary storage.  This is reserved to the person using the scope.
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* Network:
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** To local network: 1x Dual 10GE SFP+ cards (2 ports)
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** To the camera computer: 10 GB Fiber direct connection -  SFP+ optical module for 10GBASE-LR
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** To gpfs RAID: inifiniband - ConnectX-3 VPI adapter card, dual-port QSFP, FDR IB (56Gb/s) / 40GigE, PCIe 3.0 x8 8GT/s
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* CPU: 2U Dual 2.1GHz Intel E5-2620 v4
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* Memory: 128GB memory (8x 16GB)
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* Storage: 9x 8TB 7.2K SATA drives, 1x 120GB SSD drive
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* GPU: 2x NVIDIA PNY GeForce GTX 1080.
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h3. DE20
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Direct Electron frame alignment program runs on CPUs. It has its dedicated computer.
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* CPU: 32 x Intel Xeon E5-2640 v2 @ 2.00 GHz
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* Memory: 132 GB RAM
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* Job scheduler Torque installed.
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h2. Primary Storage
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GPFS 1 pB
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h2. [[SEMC file storage policy]]