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Amber Herold, 04/23/2010 09:07 AM
Do and Do Not in leginon¶
- DO NOT use low dose mode unless "Manual" application is used.
- DO wait for > 1/2 hr, with scope in high mag HM mode, after LM atlas is
acquired.
- DO check and align microscope in both HM and LM mode well.
- DO wait for targets to finish processing and Do refresh them on the atlas in "Square
Targeting" Node before selecting and submitting new ones.
- DO use the "Pause" tool in nodes such as "Square," "Hole," and in Maunal Check window
of "Z Focus" and "Focus"to pause MSI and adjust microscopy parameters directly.
- DO NOT move the stage at the microscope while Leginon pauses for user verification of
"Exposure Targeting" without using Queuing option on the node. If you do so, click the
"Declare Drift" button " ! " in "Target Adjustment" node.
Updated by Amber Herold over 14 years ago · 1 revisions