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Dose (Camera Sensitivity) Calibration » History » Version 1

Amber Herold, 04/22/2010 04:55 PM

1 1 Amber Herold
h1. Dose (Camera Sensitivity) Calibration
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Dose Calibration is a calibration of CCD sensitivity. This calibration is required only
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once at each high tension per instrument. It can be used for three purposes:
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*  To obtain a scaling factor between the scope screen current and the beam current
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measured by a standardized equipment such as a Faraday cup.
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*  To calibrate the camera sensitivity to electron.
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*  If camera sensitivity is already calibrated, the value can be entered
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directly.
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h2.  Determine the screen current/beam current scale factor
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The following uses a Faraday cup
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*  Scope> Move the stage to an empty area or a broken hole.
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*  Scope> Adjust the beam so that it passes through the measuring area of the
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Faraday cup.
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*  Scope> Choose a magnification so that the same beam will pass within the main
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screen, too.
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*  Leginon/Dose/Dose Calibrator> Beam Diameter = 0.16 m for Tecnai main
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screen.
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*  Leginon/Dose/Dose Calibrator> Enter Screen Current to Beam Current Scale
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Factor as 1 as a starting point.
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*  scope> Optionally lower the screen. Dose Calibrator will automatically do this
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if the user forgets.
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*  Leginon/Dose/Dose Calibrator> Click Measure Dose Rate. The Dose Rate, Beam
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Current, and Screen Magnification will appear in the corresponding fields after they
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have been calculated.
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*  Leginon/Dose/Dose Calibrator> Adjust the Screen Current to Beam Current Scale
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Factor so that the displayed beam current is equal to that of the Faraday cup
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measurement. NRAMM Tecnai's value is 0.88.
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h2.  Calibrate the camera sensitivity
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*  Scope> Move the stage to an empty area or a broken hole.
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*  Scope> Expand the beam so that it covers the main screen and illuminate it
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uniformly.
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*  Leginon/Dose/Settings> Configure camera with overide preset activated.
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*  Leginon/Dose/Dose Calibrator>
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Beam Diameter = 0.16 m for the Tecnai's main viewing screen.
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Screen Current to Beam Current Scale Factor = (as determined above or 1 to assume
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good calibration by the microscope manufacturer)
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*  scope> Optionally lower the screen. Dose Calibrator will automatically do this
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if the user forgets.
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*  Leginon/Dose/Dose Calibrator> Click Measure Dose Rate. The Dose Rate, Beam
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Current, and Screen Magnification will appear in the corresponding fields after they
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have been calculated.
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*  Leginon/Dose/Dose Calibrator> Without changing the beam intensity, click on
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"Calibrate". An image is acquired by Leginon.
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*Leginon will automatically lift the main viewing screen on the microscope. Some
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users report that the function does not work on their microscopes. Do it manually if
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the automatic function does not work.
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*  The calculated CCD sensitivity (counts / electron) will display once the
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calculation is completed.
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h2.  Enter pre-determined camera sensitivity value
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*  Leginon/Dose/Dose Settings/Camera Sensitivity> Enter Sensitivity values in
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counts per electron.
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*  Leginon/Dose/Dose Settings/Camera Sensitivity> Click on Save.
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|_.Preset|_.magnification|
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|*|any|