Leginon

h1. FEI TecnaiTitan installation specifics

h2. Install win32 version of python subpackages

Because all interaction with the scope uses COM interface that is only accessible through 32-bit architecture, python running Leginon component is the 32-bit version.  Use the 32-bit Windows files (those marked as win32 and certainly not the ones says 64) in http://emg.nysbc.org/projects/leginon/files for these.

*Talos has a 64-bit Python pre-installed unde default python location C:\Python27.  Therefore, we need to name our installation with another name, such as C:\Python27-win32.*

Here is the full list

{{include(Windows_32_External_Package_Installers_Common)}}

{{include(Comtypes_installer)}}

h2. Required scripting from FEI

|TEM Scripting or Tecnai Scripting|Request this when purchasing the microscope|Required|

|TomMoniker Scripting|Should come with TEM Scripting|Required|

|Low Dose Server Library|Should come with the microscope if low dose kit was purchased|Required only if you want to use low dose kit with Leginon's Manual Application|

h3. Run checkcom.py

* From a command line window:

<pre>cd C:\python27\Lib\site-packages\pyscope

C:\python27\python.exe checkcom.py</pre>

* The output should at least find typelib for

<pre>

TEM Scripting

</pre>

and

<pre>

TOM Moniker

</pre>

h2. instruments.cfg

cs is *constant for sperical abberation*

For Tecnai series:

<pre>

[TEM]

class: tecnai.Tecnai

cs: 2.0e-3

</pre>

For Titan:

<pre>

[TEM]

class: tecnai.Tecnai

cs: 2.7e-3

</pre>

For Krios (different compustage)

<pre>

[TEM]

class: tecnai.Krios

cs: 2.7e-3

</pre>

For Krios EF mode, treated as a separate instrument. i.e. add

<pre>

[TEM]

class: tecnai.EFKrios

cs: 2.7e-3

</pre>

For Arctica, (Version 3.3)

<pre>

[TEM]

class: tecnai.Arctica

cs: 2.7e-3

</pre>

For Arctica, (older version)

use Krios class is fine.  Just change cs value.

h2. Testing with pyscope

For Tecnai series In python command

<pre>

from pyscope import tecnai

t = tecnai.Tecnai()

t.getMagnification()

</pre>

For Krios

<pre>

from pyscope import tecnai

t = tecnai.Krios()

t.getMagnification()

</pre>

For Krios with EF-TEM mode, add also

<pre>

from pyscope import tecnai

t = tecnai.EFKrios()

t.getMagnification()

</pre>

For Arctica (3.3 and above) (Use Krios instruction for 3.2 version)

<pre>

from pyscope import tecnai

t = tecnai.Arctica()

t.getMagnification()

</pre>

You should get the current magnification at the microscope when the main viewing screen is up

h2. Trouble Shooting

[[unable to initializ Tecnai interface error]]

h2. Programs to open before Leginon Client: None