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FEI TecnaiTitan installation specifics » History » Revision 18

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Anchi Cheng, 03/13/2017 04:35 PM


FEI Tecnai Titan installation specifics

Install win32 version of python subpackages

Because all interaction with the scope uses COM interface that is only accessible through 32-bit architecture, python running Leginon component is the 32-bit version. Use the 32-bit Windows files (those marked as win32 and certainly not the ones says 64) in http://emg.nysbc.org/projects/leginon/files for these.

Talos has a 64-bit Python pre-installed unde default python location C:\Python27. Therefore, we need to name our installation with another name, such as C:\Python27-win32.

Here is the full list

Program package web site local copy of win32 installer
Python 2.7.16 https://www.python.org python-2.7.16.msi
wxPython 2.5.2.8 or newer https://www.wxpython.org wxPython2.8-win32-unicode-2.8.12.1-py27.exe
Python Imaging Library (PIL) 1.1.4 or newer http://www.pythonware.com/products/pil/ PIL-1.1.7.win32-py2.7.exe
NumPy (use only from our file to match compiled numextension numpy-1.7.0-win32-python2.7.exe
SciPy 0.5.1 or newer http://www.scipy.org scipy-0.11.0-win32-superpack-python2.7.exe
Leginon 3.5 or before
MySQL Python client 1.2 or newer http://sourceforge.net/projects/mysql-python MySQL-python-1.2.4b4.win32-py2.7.exe
Leginon 3.6 and up
pyMySQL 0.10.1 (not higher) https://pypi.org/project/PyMySQL/ pyMySQL-0.10.1-py2.py3-none-any.whl
Program package web site local copy of win32 installer
comtypes 0.6.2 http://sourceforge.net/projects/comtypes" comtypes-0.6.2.win32.exe

Required scripting from FEI

TEM Scripting or Tecnai Scripting Request this when purchasing the microscope Required
TomMoniker Scripting Should come with TEM Scripting Required
Low Dose Server Library Should come with the microscope if low dose kit was purchased Required only if you want to use low dose kit with Leginon's Manual Application

Run checkcom.py

  • From a command line window:
    cd C:\python27\Lib\site-packages\pyscope
    C:\python27\python.exe checkcom.py
  • The output should at least find typelib for
    TEM Scripting
    

    and
    TOM Moniker
    

instruments.cfg

cs is constant for sperical abberation

For Tecnai series:

[TEM]
class: tecnai.Tecnai
cs: 2.0e-3

For Titan:

[TEM]
class: tecnai.Tecnai
cs: 2.7e-3

For Krios (different compustage)

[TEM]
class: tecnai.Krios
cs: 2.7e-3

For Krios EF mode, treated as a separate instrument. i.e. add

[TEM]
class: tecnai.EFKrios
cs: 2.7e-3

Testing with pyscope

For Tecnai series In python command

from pyscope import tecnai
t = tecnai.Tecnai()
t.getMagnification()

For Krios

from pyscope import tecnai
t = tecnai.Krios()
t.getMagnification()

For Krios with EF-TEM mode, add also

from pyscope import tecnai
t = tecnai.EFKrios()
t.getMagnification()

You should get the current magnification at the microscope when the main viewing screen is up

Trouble Shooting

unable to initializ Tecnai interface error

Programs to open before Leginon Client: None

Updated by Anchi Cheng over 7 years ago · 18 revisions