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Anchi Cheng, 03/13/2017 04:35 PM
FEI Tecnai Titan installation specifics¶
Install win32 version of python subpackages¶
Because all interaction with the scope uses COM interface that is only accessible through 32-bit architecture, python running Leginon component is the 32-bit version. Use the 32-bit Windows files (those marked as win32 and certainly not the ones says 64) in http://emg.nysbc.org/projects/leginon/files for these.
Talos has a 64-bit Python pre-installed unde default python location C:\Python27. Therefore, we need to name our installation with another name, such as C:\Python27-win32.
Here is the full list
Program | package web site | local copy of win32 installer |
Python 2.7.16 | https://www.python.org | python-2.7.16.msi |
wxPython 2.5.2.8 or newer | https://www.wxpython.org | wxPython2.8-win32-unicode-2.8.12.1-py27.exe |
Python Imaging Library (PIL) 1.1.4 or newer | http://www.pythonware.com/products/pil/ | PIL-1.1.7.win32-py2.7.exe |
NumPy | (use only from our file to match compiled numextension | numpy-1.7.0-win32-python2.7.exe |
SciPy 0.5.1 or newer | http://www.scipy.org | scipy-0.11.0-win32-superpack-python2.7.exe |
MySQL Python client 1.2 or newer | http://sourceforge.net/projects/mysql-python | MySQL-python-1.2.4b4.win32-py2.7.exe |
pyMySQL 0.10.1 (not higher) | https://pypi.org/project/PyMySQL/ | pyMySQL-0.10.1-py2.py3-none-any.whl |
Program | package web site | local copy of win32 installer |
comtypes 0.6.2 | http://sourceforge.net/projects/comtypes" | comtypes-0.6.2.win32.exe |
Required scripting from FEI¶
TEM Scripting or Tecnai Scripting | Request this when purchasing the microscope | Required |
TomMoniker Scripting | Should come with TEM Scripting | Required |
Low Dose Server Library | Should come with the microscope if low dose kit was purchased | Required only if you want to use low dose kit with Leginon's Manual Application |
Run checkcom.py¶
- From a command line window:
cd C:\python27\Lib\site-packages\pyscope C:\python27\python.exe checkcom.py
- The output should at least find typelib for
TEM Scripting
andTOM Moniker
instruments.cfg¶
cs is constant for sperical abberation
For Tecnai series:
[TEM] class: tecnai.Tecnai cs: 2.0e-3
For Titan:
[TEM] class: tecnai.Tecnai cs: 2.7e-3
For Krios (different compustage)
[TEM] class: tecnai.Krios cs: 2.7e-3
For Krios EF mode, treated as a separate instrument. i.e. add
[TEM] class: tecnai.EFKrios cs: 2.7e-3
Testing with pyscope¶
For Tecnai series In python command
from pyscope import tecnai t = tecnai.Tecnai() t.getMagnification()
For Krios
from pyscope import tecnai t = tecnai.Krios() t.getMagnification()
For Krios with EF-TEM mode, add also
from pyscope import tecnai t = tecnai.EFKrios() t.getMagnification()
You should get the current magnification at the microscope when the main viewing screen is up
Trouble Shooting¶
unable to initializ Tecnai interface error
Programs to open before Leginon Client: None¶
Updated by Anchi Cheng over 7 years ago · 18 revisions