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Anchi Cheng, 10/31/2017 11:35 PM
FEI Tecnai Titan installation specifics¶
Install win32 version of python subpackages¶
Use the 32-bit Windows files (those marked as win32 and certainly not the ones says 64) in http://emg.nysbc.org/projects/leginon/files for these.
Here is the full list
Program | package web site | local copy of win32 installer |
Python 2.7.16 | https://www.python.org | python-2.7.16.msi |
wxPython 2.5.2.8 or newer | https://www.wxpython.org | wxPython2.8-win32-unicode-2.8.12.1-py27.exe |
Python Imaging Library (PIL) 1.1.4 or newer | http://www.pythonware.com/products/pil/ | PIL-1.1.7.win32-py2.7.exe |
NumPy | (use only from our file to match compiled numextension | numpy-1.7.0-win32-python2.7.exe |
SciPy 0.5.1 or newer | http://www.scipy.org | scipy-0.11.0-win32-superpack-python2.7.exe |
MySQL Python client 1.2 or newer | http://sourceforge.net/projects/mysql-python | MySQL-python-1.2.4b4.win32-py2.7.exe |
pyMySQL 0.10.1 (not higher) | https://pypi.org/project/PyMySQL/ | pyMySQL-0.10.1-py2.py3-none-any.whl |
Program | package web site | local copy of win32 installer |
comtypes 0.6.2 | http://sourceforge.net/projects/comtypes" | comtypes-0.6.2.win32.exe |
Required scripting from FEI¶
TEM Scripting or Tecnai Scripting | Request this when purchasing the microscope | Required |
TomMoniker Scripting | Should come with TEM Scripting | Required |
Low Dose Server Library | Should come with the microscope if low dose kit was purchased | Required only if you want to use low dose kit with Leginon's Manual Application |
Run checkcom.py¶
- From a command line window:
cd C:\python27\Lib\site-packages\pyscope C:\python27\python.exe checkcom.py
- The output should at least find typelib for
TEM Scripting
andTOM Moniker
If you have Low Dose Kit
Tecnai Low Dose Kit
You will only find Tecnai Exposure Adaptor (Scripting for film exposure) if you ask FEI for it.
instruments.cfg¶
cs is constant for sperical abberation
For Tecnai series:
[TEM] class: tecnai.Tecnai cs: 2.0e-3
For Titan:
[TEM] class: tecnai.Tecnai cs: 2.7e-3
For Krios (different compustage)
[TEM] class: tecnai.Krios cs: 2.7e-3
For Krios EF mode, treated as a separate instrument. i.e. add
[TEM] class: tecnai.EFKrios cs: 2.7e-3
Testing with pyscope¶
For Tecnai series In python command
from pyscope import tecnai t = tecnai.Tecnai() t.getMagnification()
For Krios
from pyscope import tecnai t = tecnai.Krios() t.getMagnification()
For Krios with EF-TEM mode, add also
from pyscope import tecnai t = tecnai.EFKrios() t.getMagnification()
You should get the current magnification at the microscope when the main viewing screen is up
Trouble Shooting¶
unable to initializ Tecnai interface error
Programs to open before Leginon Client: None¶
Updated by Anchi Cheng about 7 years ago · 20 revisions