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FEI TecnaiTitan installation specifics » History » Revision 24

Revision 23 (Anchi Cheng, 01/29/2018 04:05 PM) → Revision 24/27 (Anchi Cheng, 01/29/2018 04:15 PM)

h1. FEI Tecnai Titan installation specifics 

 h2. Install win32 version of python subpackages 

 Use the 32-bit Windows files (those marked as win32 and certainly not the ones says 64) in http://emg.nysbc.org/projects/leginon/files for these. 

 Here is the full list 
 {{include(Windows_32_External_Package_Installers_Common)}} 
 {{include(Comtypes_installer)}} 

 h2. Required scripting from FEI 

 |TEM Scripting or Tecnai Scripting|Request this when purchasing the microscope|Required| 
 |TomMoniker Scripting|Should come with TEM Scripting|Required| 
 |Low Dose Server Library|Should come with the microscope if low dose kit was purchased|Required only if you want to use low dose kit with Leginon's Manual Application| 

 

 h3. Run checkcom.py 

 * From a command line window: 
 <pre>cd C:\python27\Lib\site-packages\pyscope 
 C:\python27\python.exe checkcom.py</pre> 

 * The output should at least find typelib for 
 <pre> 
 TEM Scripting 
 </pre> 
 and 
 <pre> 
 TOM Moniker 
 </pre> 

 If you have Low Dose Kit 
 <pre> 
 Tecnai Low Dose Kit 
 </pre> 

 You will only find Tecnai Exposure Adaptor (Scripting for film exposure) if you ask FEI for it. 

 

 h2. instruments.cfg 

 cs is *constant for sperical abberation* 

 For Tecnai series: 
 <pre> 
 [TEM] 
 class: fei.Tecnai 
 cs: 2.0e-3 
 </pre> 

 For Halo (Titan column with tecnai compustage): 
 <pre> 
 [TEM] 
 class: fei.Halo 
 cs: 2.7e-3 
 </pre> 

 For Krios (different compustage) 
 <pre> 
 [TEM] 
 class: fei.Krios 
 cs: 2.7e-3 
 </pre> 

 For Krios EF mode, treated as a separate instrument. i.e. add 
 <pre> 
 [TEM] 
 class: fei.EFKrios 
 cs: 2.7e-3 
 </pre> 

 

 h2. Setup fei.cfg 

 [[setup fei.cfg]] 

 h2. Testing with pyscope 

 For Tecnai series In python command 
 <pre> 
 from pyscope import fei 
 t = fei.Tecnai() 
 t.getMagnification() 
 </pre> 

 For Krios 
 <pre> 
 from pyscope import fei 
 t = fei.Krios() 
 t.getMagnification() 
 </pre> 

 For Krios with EF-TEM mode, add also 
 <pre> 
 from pyscope import fei 
 t = fei.EFKrios() 
 t.getMagnification() 
 </pre> 

 You should get the current magnification at the microscope when the main viewing screen is up 

 

 h2. Trouble Shooting 

 [[unable to initializ Tecnai interface error]] 

 h2. Programs to open before Leginon Client: None