Gatan K2 installation and setup » History » Revision 13
Revision 12 (Anchi Cheng, 06/05/2014 10:54 PM) → Revision 13/47 (Anchi Cheng, 06/06/2014 11:25 AM)
h1. Gatan K2 unique installation and setup h2. Gatan K2 is controlled by a computer separated from the microscope Please read [[Using_Leginon_on_a_system_where_the_microscope_and_camera_are_controlled_by_different_computers]] first. h2. Extra Package and Installation * Use all amd64 version of Windows installer * SerialEM DigitalMicrograph Plug-in {{include(Install_SerialEM_DigitalMicrograph_plugin)}} h2. instruments.cfg {{include(Gatan_K2_Instruments_cfg)}} h2. Setup * Set camera configuration to 270 degree rotation and flip around the vertical axis to give the [[Leginon Image Orientation|standard Leginon orientation]]. Note that the rotation required is different if it is installed post-GIF and/or on Krios. * Set the [[Low_dose_shutter_configuration_for_Gatan_camera_in_Digital_Micrograph_program|shutter configuration in DM]] to protect the specimen when camera is not taking images. * Create the folder to store the dose fractionation raw frames. Default location is D:\\frames. * Setup [[DDD_raw_frame_file_transfer|raw frame file transfer]] from a network data server. h2. Change Flag in pyscope/dmsem.py if you have DM 2.30 version and requires flip/rotation to give the Leginon orientation. In standard python site-package installation, this file is at C:\Python27\Lib\site-packages\pyscope\demsem.py Find this line near the top: <pre> isDM230 = False </pre> Change it to <pre> isDM230 = True </pre> h2. Testing with pyscope {{include(Gatan_K2_Installation_Pyscope_Test)}} h2. Programs to open before Leginon Client: Digital Micrograph h2. See more about K2 implementation in [[Using_Gatan_K2_Summit_in_Leginon]]