Gatan K2 installation and setup » History » Revision 14
Revision 13 (Anchi Cheng, 06/06/2014 11:25 AM) → Revision 14/47 (Anchi Cheng, 07/11/2014 06:14 PM)
h1. Gatan K2 unique installation and setup
h2. Gatan K2 is controlled by a computer separated from the microscope
Please read [[Using_Leginon_on_a_system_where_the_microscope_and_camera_are_controlled_by_different_computers]] first.
h2. Extra Package and Installation
* Use all amd64 version of Windows installer
* SerialEM DigitalMicrograph Plug-in
{{include(Install_SerialEM_DigitalMicrograph_plugin)}}
h2. instruments.cfg
{{include(Gatan_K2_Instruments_cfg)}}
h2. Setup
* Set camera configuration to give the [[Leginon Image Orientation|standard Leginon orientation]]. For example, we need 270 degree rotation and flip around the vertical axis on FEI F20. to give the [[Leginon Image Orientation|standard Leginon orientation]]. Note that the rotation required is different if it is installed post-GIF and/or on Krios.
* Set the [[Low_dose_shutter_configuration_for_Gatan_camera_in_Digital_Micrograph_program|shutter configuration in DM]] to protect the specimen when camera is not taking images.
* Create the folder to store the dose fractionation raw frames. Default location is D:\\frames.
* Setup [[DDD_raw_frame_file_transfer|raw frame file transfer]] from a network data server.
h2. Change Flag in pyscope/dmsem.py if you have DM 2.30 version and requires flip/rotation to give the Leginon orientation.
In standard python site-package installation, this file is at C:\Python27\Lib\site-packages\pyscope\demsem.py
Find this line near the top:
<pre>
isDM230 = False
</pre>
Change it to
<pre>
isDM230 = True
</pre>
h2. Testing with pyscope
{{include(Gatan_K2_Installation_Pyscope_Test)}}
h2. Programs to open before Leginon Client: Digital Micrograph
h2. See more about K2 implementation in [[Using_Gatan_K2_Summit_in_Leginon]]