Leginon

h1. Gatan on Windows-32 with dmsem

dmsem module uses SEMCCD from distributed by SerialEM to communicate with Gatan software rather than tecnaiccd.dll

If using this on a computer different from the scope computer, please read [[Using_Leginon_on_a_system_where_the_microscope_and_camera_are_controlled_by_different_computers|this]] first.

h2. Extra Package and Installation

* Use all win32 version of Windows installer

* SerialEM DigitalMicrograph Plug-in

{{include(Install_SerialEM_DigitalMicrograph_plugin)}}

h2. instruments.cfg

Currently UltraScan and Orius camera are supported.

For UltraScan camera (US4000), set instruments.cfg to use GatanUltraScan class. For example:

<pre>

[us4000]

class: dmsem.GatanUltraScan

zplane: 50

height: 4096

width: 4096

</pre>

h2. Setup

!camera_configuration.png!

* Set camera configuration to give the [[Leginon Image Orientation|standard Leginon orientation]].  For example, we need flip around the vertical axis on FEI T12.  Note that the rotation required is different if it is installed post-GIF and/or on Krios.

* Set the [[Low_dose_shutter_configuration_for_Gatan_camera_in_Digital_Micrograph_program|shutter configuration in DM]] to protect the specimen when camera is not taking images.

h2. (Version 3.2) Define local configuration in dmsem.cfg

# Copy from your myami checkout pyscope/dmsem.cfg.template to C:\Program Files\myami\dmsem.cfg

# Make changes to the configured value in dmsem.cfg

Most parameters are explained above.

* *The config parser takes "'" and "\" literally*.  Therefore, do not include "'" in DM_VERION.  In addition, the path separator should be "\"

* *CAMERA_ID* is usually 0 if it is the only Gatan camera.

* *Ultrascan camera should be configured under utrascan module, and Orius camera should be configured under orius module.

For example, only UltraScan camera exists on this computer that has Digital Micrograph version 2.32.788.0, and DM camera configuration

has vertical flip but no rotation, then set the dmsem.cfg ultrascan module like this:

<pre>

[dm]

DM_VERSION = 2.32.788.0

[ultrascan]

CAMERA_ID = 0

FLIP = True

ROTATE = 0

</pre>