Grids for Calibration » History » Version 1
Amber Herold, 04/22/2010 04:45 PM
| 1 | 1 | Amber Herold | h1. Grids for Calibration |
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| 6 | The grid used for calibration should have reasonable contrast and features at multiple |
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| 7 | scale. A frozen grid is generally not convenient for the purpose since the contrast is low and |
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| 8 | can drift easily. Large protein complex negatively stained on a 200-400 mesh em grid with |
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| 9 | Quantifoil, C-falt, or home-made holey carbon support is ideal. You can even use one that is |
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| 10 | abandoned after a cryo run, just negatively stain it to improve the contrast. From our |
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| 11 | experience, small protein negatively stained on continuous carbon support oftern gives low |
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| 12 | contrast at intermediate magnification and therefore not ideal. If your Quantifoil or C-flat |
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| 13 | grid is too clean, there may be a problem of false peaks from the lattice. In this case, you |
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| 14 | can add some gold clusters such as Nanogold from Nanoprobe. |