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TEM¶
cs is the coefficient of spherical aberration in meters. Find out from TEM manufacture the proper value for your tem.
For example
[tem] class: fei.Tecnai cs: 2.0e-3
Camera¶
height and width are number of pixels based on the orientation presented in Leginon image viewer- Some Tietz camera may have an odd physical dimension. Use TCL/EMMenu program to find out the good dimension used in Tietz's own software and entered in this configuration.
For example
[camera] class: gatan.Gatan zplane: 50 height: 4096 width: 4096
zplane is a number that gives the order of camera. If a camera with low zplane value is going to acquire an image, Leginon will attempt to retract all cameras at higher zplane values.
HEIGHT and WIDTH are not shown in these examples for simplicity- One computer for both tem and digital camera:
[tem] class: fei.Tecnai cs: 2.0e-3 [camera] class: gatan.Gatan zplane: 50
- Separate computers for the two instruments: configure only the instrument reside on the particular computer
- On the computer that controls the tem:
[tem] class: fei.Tecnai cs: 2.0e-3
- On the computer that controls the digital camera:
[camera] class: gatan.Gatan zplane: 50
- On the computer that controls the tem:
- Two camera at the same plane:
- On the computer that controls the digital camera:
DE12 and DE Survey camera are retract/extend together, with the survey camera at off-axis. Therefore zplane should be set at the same value
For example,[camera1] class: de.DE12 zplane: 50 [camera2] class: de.DESurvey zplane: 50
- On the computer that controls the digital camera:
- Gatan Orius camera that is installed typically with Gatan K2/Falcon camera is technically on the same plane but is not capable of insertion unless K2 is retracted. On the other hand, K2 camera insertion includes a retraction of Orius at lower level of the function call in DM. Therefore, Orius is considered as a camera at lower zplane.
[camera1] class: dmsem.GatanK2Base zplane: 50 [camera2] class: dmsem.GatanOrius zplane: 49
Updated by Anchi Cheng almost 7 years ago ยท 17 revisions