Leginon

h1. Krios-K2 Presets used for 2.8 A proteasome reconstruction in 5 hours data collection at SEMC @ NYSBC

* Nanoprobe was used to allow smaller beam to be used for multiple targeting on 2 um hole although not essential in this case.

Tecnai Krios 300 kV high tension, XFEG, Gun Lens 4 extraction voltage 4100, 70 um C2 aperture  and 100 um objective aperture (except when obtaining grid atlas).

Gatan camera dimension 3710(w)x 3838(h) after rotation to Leginon standard.  The size of the beam on the scope main viewing screen always covers the 1.2 um hole at en preset on our scope.

Pixel size is 1.10 A at 22500x scope nominal mag.:

|Probe|_.Lens Series|Magnification:|_.Preset name:|_.Image Shift (x,y):|_.Dimension:|_.Binning:|_.Camera Dose (e/camera pixel/s):|_.Exposure Time (ms):|_.Specimen Dose Rate (e/A^2):|_.Spot Size:|_.Defocus (m):|_.C2 (um):|frame saving:|Camera|

|micro|LM|100|gr|Aligned|1024x1024|4|n/a|500|n/a|5|0.0|70|no|Ceta|

|micro|SA|400**|sq|Aligned|927x959|4|8|100|n/a|10|-2e-4|70|no|K2|

|nano|SA|1300**|hl|Aligned|927x959|4|8|200|0.004|11|-1.5e-4|70|no|K2|

|nano|SA|22500|fc|0,0|924x924|2|>=8*|500|3.3|8|-7e-7|70|no|K2|

|nano|SA|22500|fa|0,0|927x959|4|>=8*|250|1.65|8|-1.5e-6|70|no|K2|

|nano|SA|22500|en|0,0|3710x3838|1|8 e/pixel/s and ~0.7 um on the specimen|7000|46|8|(-1e-6 to -2e-6)|70|200 ms/frame|

**These two magnifications are add-on to standard SA lens series made for NYSBC Krios1. May not be available at other scopes.

* Note: If the correlation or power spectrum don't work well enough for the standard Camera Dose Rate of 10 e/pixel/s for fc and fa, you may increase it more so not to have to use longer exposure time.  The recommended value in literature is meant to give highest DQE at all sampling frequencies. Using a higher camera dose by 50% will certainly not damage the sensor.

* Note: no defocal pair was acquired in this experiment.  Therefore, no ef preset.