Leginon Main Page¶

Overview¶

Leginon is a system designed for automated collection of images from a transmission electron microscope.

Contact information:¶

Please email amisoftware@scripps.edu with any questions.

Software Installation:¶

see Complete Appion Installation

User Guide:¶

see Appion User Guide

Publications:¶

Primary Publication to Cite:¶

• Lander, G.C.; Stagg, S.M., Voss, N.R., Cheng, A., Fellmann, D., Pulokas, J., Yoshioka, C., Irving, C., Mulder, A., Lau, P.W., et al. (2009). "Appion: an integrated, database-driven pipeline to facilitate EM image processing.". Journal of Structural Biology 166: 95-102. PMID 19263523.

Additional References:¶

• Voss, N.R.; Lyumkis D, Cheng A, Lau PW, Mulder A, Lander GC, et al. (2010). "A toolbox for ab initio 3-D reconstructions in single-particle electron microscopy.". Journal of Structural Biology 169 (3): 389-98. PMID 20018246.

• Stagg, S.M.; Lander GC, Quispe J, Voss NR, Cheng A, Bradlow H, Bradlow S, Carragher B, Potter CS (2008). "A test-bed for optimizing high-resolution single particle reconstructions". Journal of Structural Biology 163 (1): 29-39. PMID 18534866.

Software Availability and Licensing Information¶

Appion is released under the Apache License, Version 2.0

View an overview of the Appion code base found in the "myami" repository.

Youtube videos:¶

• An Introduction to Appion
• TiltPicker Demonstration