Leginon

h1. Leginon Operation for HT7800

Here is an example of presets used on our HT7800 TVIPS-XF416

LaB6 filament Operated at HT 100 kV *This is limited by the current implementation to match calibration from the manufacturer.*

Unbinned image pixel size at 120kx nominal magnification is 1.76 Å

HT7800 has a few illumination (column mode)/projection presets.

We use LOWMAG, ZOOM1-HC, and ZOOM1-HR with presets involved in targeting final acquistion

* Spot Size noted here is not the same definition as what HT interface is shown as Spot number.  It corresponds to a combinatopm observation mode (HR-field or HC field) and Spot number.

|_.Magnification:|_.probe mode:|_.Preset name:|_.Image Shift (x,y):|_.Dimension:|_.Binning:|_.Beam coverage:|_.Exposure Time (ms):|_.Spot Size as seen in Leginon interface*:|_.Defocus (m):|

|300|low-mag|gr|Aligned|1024|4|max|100|1|0.0|

|1500|micro-hc|sq|Aligned|1024|4|1x CCD size|100|3|0.0|

|15000|micro-hr|hl|Aligned|1024|4|1x CCD|100|3|-5e-4|

|120000|micro-hr|fc|0,0|1024|2|~2x CCD|400|5|-5e-7|

|120000|micro-hr|fa|0,0|1024|4|~4x CCD|100|5|-2e-6|

|120000|micro-hr|en|0,0|4096|1|~4x CCD|100|5|-2e-6|