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Anchi Cheng, 07/30/2021 01:35 PM
Leginon Operation for HT7800¶
Here is an example of presets used on our HT7800 TVIPS-XF416
LaB6 filament Operated at HT 100 kV This is limited by the current implementation to match calibration from the manufacturer.
Unbinned image pixel size at 120kx nominal magnification is 1.76 Å
HT7800 has a few illumination and imaging system presets. Zoom-1 imaging system presets is what we use.
We use Column mode LOWMAG, ZOOM1-HC field, and ZOOM1-HR field. The last is used for presets involved in targeting acquiring the final images.
- Spot Size behaves the same as Spot number on Hitachi interface. However, Hitachi gui does not always change its display when we set this value at the lower level. Please check C1 and C2 lens values for the actual effect correspondance.
- Preset Cycling should always be used.
| Magnification: | probe mode: | Preset name: | Image Shift (x,y): | Dimension: | Binning: | Beam coverage: | Exposure Time (ms): | Spot Size: | Defocus (m): |
|---|---|---|---|---|---|---|---|---|---|
| 300 | low-mag | gr | Aligned | 1024 | 4 | max | 100 | 1 | 0.0 |
| 1500 | micro-hc | sq | Aligned | 1024 | 4 | 1x CCD size | 100 | 3 | 0.0 |
| 15000 | micro-hr | hl | Aligned | 1024 | 4 | 1x CCD | 100 | 3 | -5e-4 |
| 120000 | micro-hr | fc | 0,0 | 1024 | 2 | ~2x CCD | 400 | 5 | -5e-7 |
| 120000 | micro-hr | fa | 0,0 | 1024 | 4 | ~4x CCD | 100 | 5 | -2e-6 |
| 120000 | micro-hr | en | 0,0 | 4096 | 1 | ~4x CCD | 100 | 5 | -1e-6 |
The screenshot below shows what each Hitachi panel looks like in at en preset.
Updated by Anchi Cheng over 3 years ago · 5 revisions