Leginon

h2. Make DDD movie stack

The following section comes from Appion Manual

{{include(appion:Direct_Detector_Frame_Processing)}}

h2. Making Gain/Dark correction one image at a time without saving results to databases

Two scripts are attached here.  They use Leginon infrastructure to locate references and do gain/dark correction and bad pixel/column/row correction.  It, however, does not involve saving results to the leginon/appion databases.  These are good for testing of add your own idea to it.

*correctframes.py* let you correct a range of raw frames in an image and output as mrc file.

*correctstack.py* let you correct each frame in the specified summed image and output at 2D image stack in mrc format.

type:

<pre>

python correctframes.py

</pre>

for the options

{{include(Make movie stack with dark/bright/norm/gain references yourself without Leginon/Appion libraries)}}