Make DDD movie stack » History » Revision 5
Revision 4 (Anchi Cheng, 08/26/2014 05:43 PM) → Revision 5/17 (Anchi Cheng, 08/26/2014 05:47 PM)
h2. Make DDD movie stack
The following section comes from Appion Manual
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h2. Making Gain/Dark correction Run one image at a time without saving results uploading aligned to Leginon/Appion databases
Two scripts using Leginon infrastructure to locate references and do gain/dark correction but not involving saving results to the leginon/appion databases are attached here
*correctframes.py* let you correct a range of raw frames in an image and output as mrc file.
*correctstack.py* let you correct each frame in the specified summed image and output at 2D image stack in mrc format.
type:
<pre>
python correctframes.py
</pre>
for the options
h2. Do it yourself
If you want to do everything yourself, you can use the python script
"getReferences.py":http://emg.nysbc.org/projects/leginon/repository/changes/branches/myami-3.1/leginon/getReferences.py
to find the location and filenames of the references and work on them yourself. *Beware that the fames are often in different orientation from the reference images in Leginon*