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Make DDD movie stack » History » Revision 7

Revision 6 (Anchi Cheng, 08/26/2014 05:54 PM) → Revision 7/17 (Anchi Cheng, 08/26/2014 05:57 PM)

h2. Make DDD movie stack 

 The following section comes from Appion Manual 

 {{include(appion:Direct_Detector_Frame_Processing)}} 

 h2. Making Gain/Dark correction one image at a time without saving results to databases 

 Two scripts are attached here.    They use using Leginon infrastructure to locate references and do gain/dark correction and bad pixel/column/row correction.    It, however, does but not involve involving saving results to the leginon/appion databases.    These databases are good for testing of add your own idea to it. attached here 

 *correctframes.py* let you correct a range of raw frames in an image and output as mrc file. 
 *correctstack.py* let you correct each frame in the specified summed image and output at 2D image stack in mrc format. 

 type: 
 <pre> 
 python correctframes.py 
 </pre> 
 for the options 

 h2. Do it yourself 

 If you want to do everything yourself, you can use the python script 

 "getReferences.py":http://emg.nysbc.org/projects/leginon/repository/changes/branches/myami-3.1/leginon/getReferences.py 

 to find the location and filenames of the references and work on them yourself. 

 *Beware that the fames are often in different orientation from the reference images in Leginon* 

 If there are no bad pixel/column/row on your camera that you included in your correction plan, you can feed the properly rotated/flipped references to the drift correction program by Xueming Li.