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Manual Acquisition » History » Revision 2

Revision 1 (Amber Herold, 04/23/2010 10:00 AM) → Revision 2/3 (Amber Herold, 04/27/2010 02:40 PM)

h1. Manual Acquisition 



 Manual Acquisition is a node that allows the electron microscopist to acquire CCD images 
 manually while at the microscope. This node essentially creates a button to acquire images 
 manually with Leginon and gives all the benefits of the Leginon database and image storage. 



 Required bindings: None 





 h2.    Toolbar 





 *    Settings = Adjust the manual image acquisition parameters. 


 *    Grids = Select the current grid.(Not used without Project Database) 


 *    Acquire = Acquire a current image. 


 *    Continuous Acquire = Click this to begin acquiring images continuously. 


 *    Abort = Stop the Continuous Acquire function. 









 h2.    Settings 





 *    Camera Configuration = Select the parameters that describe how the image is 
 acquired. 

 - Dimension = image x,y pixel dimension 

 - Offset = x,y offset from top left corner of full CCD image 

 - Binning = x,y image binning 

 - pre-configured image sizes 

 - Custom = customize image dimension, offset, and binning 

 - Exposure time (ms) = CCD and specimen expsosure time 


 *    Correct image = When enabled, the raw CCD image will be corrected with Bright and 
 Dark CCD reference images 


 *    Save image to database = When enabled, the acquired CCD image will be 
 automatically stored in the database. All microscope and camera information will be 
 stored for each image. 


 *    Loop pause "30" seconds = The amount of time the Continuous Acquire function 
 waits before acquiring the next image. 


 *    Label = Enter a label to be inserted in the image file name (in addition to the 
 normal Leginon file name).* 


 *    Main Screen: Up before acquire = When enabled, Leginon will raise the 
 microscope's main viewing screen. 


 *    Main Screen: Down after acquire = When enabled, Leginon will lower the 
 microscope's main viewing screen after an image has been acquired. 


 *    Low Dose: 

 - Use low dose = When enabled, Leginon will switch the microscope from focus to 
 exposure mode of the Low Dose kit to acquire the image and then return to focus 
 mode. 

 - Low Dose pause "4" seconds = Wait this amount of time before acquiring an image 
 when using Low Dose on the microscope and acquiring images with the Acquire feature in 
 this node. Increase the time if the microscope does not change modes fast 
 enough. 






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