Manual Focusing Tool¶

Manual Focusing can be activated either by enabling "Manual_before/Manual_after" steps or directly when leginon is idle using (Z) Focus/Toolbar/MF.

• Manual_before/Manual_after focus sequence:
  • The preset used is set inside focus sequence settings
• Manual Focus Tool
  • When using Manual Focus Tool directly, the preset is set by the preset named "manual focus tool preset" found also in focuser node settings.

• MF> The FFT should show visible and isotropic Thon ring according to the fc preset defocus.

• MF/Toolbar> Make sure the move type is "Defocus" and the set value in the box is 0 m then click "Set instrument" botton . This will set the defocus to the current 0.

• MF> The perfect autofocus result is that the FFT indicates that the scope is at Gaussian focus.

• For relative movement, press "+" or "-". The step size is determined in MF/Toolbar/Settings> You can also left-click on the first node of the power spectrum displayed to change the step size.

• To reset defocus to zero at the new focus, press the so-named button .

• Clicking the Stop button exits the manual check.