Mosaic Click Target Finder » History » Version 5
Amber Herold, 06/25/2010 12:28 PM
| 1 | 1 | Amber Herold | h1. Mosaic Click Target Finder |
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| 3 | 5 | Amber Herold | Mosaic Click Target Finder is a type of TargetFinder Node. It handles the loading, save, displaying and target selection of a list of images and and their composite. |
| 4 | 1 | Amber Herold | |
| 5 | 5 | Amber Herold | *Required bindings for Mosaic Click Target Finder Node:* |
| 6 | 1 | Amber Herold | |
| 7 | 5 | Amber Herold | Grid Targeting - (ImageTargetListPublishEvent) - > AcquisitionNodeAlias |
| 8 | AcquisitionNodeAlias- (AcquisitionImagePublishEvent) -> MosaicClickTargetFinder |
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| 9 | MosaicClickTargetFinder - (ImageTargetListPublishEvent) -> AcquisitionNodeAlias |
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| 10 | ClickTargetFinder - (ReferenceTargetListPublishEvent) -> AlignZeroLossPeak or MeasureDose |
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| 11 | 1 | Amber Herold | |
| 12 | 3 | Eric Hou | |
| 13 | 5 | Amber Herold | *Required bindings for MosaicClickTargetFinder Node if Reference Targets are used:* |
| 14 | 1 | Amber Herold | |
| 15 | 5 | Amber Herold | MosaicClickTargetFinder - (ReferenceTargetListPublishEvent) -> AlignZeroLossPeak or MeasureDose |
| 16 | 1 | Amber Herold | |
| 17 | h2. Toolbar |
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| 19 | 3 | Eric Hou | * Tiles = Choose The Mosaic Tiles label (aka atlas label) to display. Mosaic Tiles are a list of images collected by an Acquisition node upstream. |
| 20 | 1 | Amber Herold | |
| 21 | 3 | Eric Hou | * Mosaic = Select how large the mosaic atlas image will be and what calibration is used to create it. |
| 22 | 1 | Amber Herold | |
| 23 | 3 | Eric Hou | * Refresh = Refresh the "Mosaic" selector to the current choices in the database. |
| 24 | 1 | Amber Herold | |
| 25 | 3 | Eric Hou | * Show Position = When clicked, the current microscope position (orange crosshair) will be updated on the atlas image. |
| 26 | 1 | Amber Herold | |
| 27 | 3 | Eric Hou | * Find Squares = An automatic algorithm for detecting squares on the atlas image. |
| 28 | 1 | Amber Herold | |
| 29 | * Submit Targets = Submit the selected squares for processing. |
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| 31 | h2. Tiles |
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| 33 | 3 | Eric Hou | * Load tiles from mosaic = Choose The Mosaic Tiles label (aka atlas label) to display. Mosaic Tiles are a list of images collected by an Acquisition node upstream. |
| 34 | 1 | Amber Herold | |
| 35 | 3 | Eric Hou | * Reset = Clears the loaded list in the Mosaic Click Target Finder, and therefore clears the mosaic image shown. |
| 36 | 1 | Amber Herold | |
| 37 | h2. Mosaic Settings |
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| 39 | 3 | Eric Hou | * Calibration parameter (stage position | image shift | modeled stage position) = Select the type of calibration used to create the atlas. Stage Position should be used. This determines how the node creates the pixel shifts for each tile image in order to "paste" it to the right position of the mosaic image. Each acquired image in the database has the full information on its stage position and image shift. This pull-down selector determines which items the subnode look into for the conversion into pixel shift. |
| 40 | 1 | Amber Herold | |
| 41 | 3 | Eric Hou | * Scale image to "1000" pixels in largest dimension = When enabled, the mosaic atlas will be scaled to this image size. |
| 42 | 1 | Amber Herold | |
| 43 | 3 | Eric Hou | * Mosaic Image (Create | Save) = Create and save the large mosaic atlas to the database. |
| 44 | 1 | Amber Herold | |
| 45 | h2. Image Control Panel |
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| 46 | |||
| 47 | * acquisition = Use this to select NEW targets shown in a greeen crosshair. |
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| 48 | |||
| 49 | 3 | Eric Hou | * done = The done targets (squares already visited) will be shown with a red crosshair. |
| 50 | 1 | Amber Herold | |
| 51 | 3 | Eric Hou | * position = The current position of the microscope relative to the grid is shown with an orange crosshair. |
| 52 | 1 | Amber Herold | |
| 53 | * image = The atlas image is shown. |
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| 55 | * Filtered = This is used for the automatic square selection algorithm |
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| 57 | * Thresholded = This is also used the the automatic square selection algorithm |
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| 58 | 2 | Amber Herold | |
| 59 | |||
| 60 | ______ |
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| 61 | |||
| 62 | [[Matrix Calibrator|< Matrix Calibrator]] | [[Mosaic Target Maker|Mosaic Target Maker >]] |
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| 63 | |||
| 64 | ______ |