Project

General

Profile

Actions

Pausing and Aborting during data collection » History » Revision 2

« Previous | Revision 2/11 (diff) | Next »
Amber Herold, 04/27/2010 02:12 PM


Pausing and Aborting during data collection

Users may need to pause leginon data collection when filling cryo-stage and cold trap or
making adjustment to image or beam shift of a preset or even perform certain microscopy
alignment. Pausing is possible when leginon is waiting for user input at target finder level.
Alternatively, pausing can be made in any acquisition node with the pause tool.

The pause tool (as well as the abort tool), when clicked, does not interrupt acquisition
until the next possible point that is at the end of an acquisition sequence for a target. When
the play tool is clicked at a later point of time, leginon should move the stage and image
shift to that defined by the next image. Here are some examples of the valid pausing
points

  • At the end of an acquisition sequence for a target in the middle of a target list
    that may also be one of the queued list.
  • At the end of an acquisition sequence for a target at the end of a target list that
    may also be one of the queued list.
  • Before a focus target is repeated after DriftManager is done monitoring drift.

Currently, you can not pause at the following points:

  • Within the preset list of an acquisition target
  • Before any target is processed at the node

Pausing in MSI application

For those using MSI-type of application, we recommand the following pausing
point:

Case 1: Exposure Targets are NOT queued

  • At Hole or Subsquare (MSI-Raster) node, click on the "Pause" tool any time and it
    will pause before the start of next hole in the same square.

Case 2: Exposure Targets are queued

  • In Exposure node and click on the "Pause" tool while the last target is being
    acquired. Leginon will then pause at the end of the exposure sequence.

< Do and Do Not in leginon | General operation problems >


Updated by Amber Herold about 14 years ago · 2 revisions