Leginon

h1. Presets for MSI-RCT applications

The main difference between MSI-RCT applications and other MSI's is that a low

magnification image is needed for the target transformation algorithm to reliably find

features to match between tilts. Two strategies has be used with success.

h2. 1st strategy:

The sq preset is set at a magnification in the HM mode so that hysteresis is minimized.

and the hl preset is eliminated  The "sq" preset is used both at "Square" and "Centered

Square" nodes.

*Presets and Magnification for a typical MSI-RCT*

|_.Preset|_.magnification|_.defocus (m)|

|gr|120|0|

|sq|1700|-2.5e-4|

|fc,fa,en|50000|(what you need)|

h2. 2nd strategy:

The sq preset is set at the usual LM magnification. The hl preset is set at the low end

of HM mode. The "sq" preset is at "Square" node and "hl" preset is used at "Centered Square"

nodes.

*Presets and Magnification for a typical MSI-RCT*

|_.Preset|_.magnification|_.defocus (m)|

|gr|120|0|

|sq|550|-2e-3|

|hl|1700|-2.5e-4|

|fc,fa,en|50000|(what you need)|

_see also [[RCT run protocol from a user]]_

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[[RCT node set-up|< RCT node set-up]] | [[RCT calibration|RCT calibration need >]]

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