RCT node set-up¶

Testing feature finding algorithm¶

The default values in the RCT node may not work for your sample, trials should be made
to optimize the parameters. The good parameters should find hundreds, but not thousands of
features in your untilted image for good results.

• Presets Manager> send the preset used in "Centered Square" to scope at the center
  of a square.

• RCT/toolbar> Click on the "acquire" button at the far right to acquire a test image
  of which the feature finding algorithm will be applied after the acquisition.

• RCT> Check the result of the numbers of feature found. A few hundred but not
  thousands would be a good settings.

• RCT/Settings> Adjust Min Feature Size (most sensitive) and/or Max Feature Size or
  the filters and then repeat 2 and 3 to get better results.

• Target templates are applied to the valid raster points to create the final
  acquisition and focus targets. The template can be constant, meaning that it is defined
  relative to the image coordinate. The template can also be convolved with the raster
  points which will create multiple targets, each defined relative to the coordinate of
  the valid raster points.

• Min/Max Feature Size: if < 1, it means faction of the image area. If > 1,
  number of pixels.

• List of Tilts to Collect are in the order of data collection

• RCT node has its own drift monitoring after each tilt at the transformed focus
  target position. Therefore, drift threshold and drift preset need to be defined.

< Summary of this application | Presets for MSI-RCT applications >