Running "Manual" Application » History » Version 1
Amber Herold, 04/22/2010 04:41 PM
| 1 | 1 | Amber Herold | h1. Running "Manual" Application |
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| 5 | * Leginon/Manual> Open the "Settings" tool !http://emg.nysbc.org/software/leginon/images/icons/settings.png! and configure the settings: |
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| 8 | Important-Use low dose kit option should be set to yes if using the low dose kit on Tecnai. Low Dose Pause Time need to be set to 5 sec or more to complete the mode change. |
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| 11 | Recommandations- |
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| 14 | Acquisition/Camera Configuration- use one of the preconfigured selection for binning and size |
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| 17 | Correct image=yes if dark and bright images of the camera configuration exist. |
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| 20 | Save image to the database=yes. |
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| 23 | * Leginon/manual/Settings> Save and close the settings by pressing "OK" button. |
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| 26 | * Adjust the exposure time in the setting and/or beam size/spot size on the scope to achieve the desired dose and coverage in exposure mode, if low-dose kit is used. |
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| 29 | * Microscope> Search the grid for interested area and focus as normally performed. |
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| 32 | * Microscope> If low dose mode is used, leave TEM in Focus model. Leave the screen at up position if "Main Screen/Up before acquire" is not checked in the settings. No need to put on beam blank. |
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| 35 | * Leginon/manual> Click on the "Acquire" tool !http://emg.nysbc.org/software/leginon/images/icons/acquire.png! to acquire the image. |
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| 38 | * Microscope> If low dose mode is used, Leginon will force the pre-specimen shutter close, switch the scope to exposure mode to acquire the image and then switch back to the original mode. |